Toward automatic reconstruction of a highly resolved tree of life

被引:1154
作者
Ciccarelli, FD
Doerks, T
von Mering, C
Creevey, CJ
Snel, B
Bork, P
机构
[1] European Mol Biol Lab, D-69012 Heidelberg, Germany
[2] European Inst Oncol, Dept Expt Oncol, I-20141 Milan, Italy
[3] Fdn Italiana Ric Cancro, Inst Mol Oncol Fdn, I-20139 Milan, Italy
[4] Radboud Univ Nijmegen Med Ctr, Nijmegen Ctr Mol Life Sci, Ctr Mol & Biomol Informat, NL-6525 ED Nijmegen, Netherlands
[5] Max Delbruck Ctr, D-13092 Berlin, Germany
关键词
D O I
10.1126/science.1123061
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We have developed an automatable procedure for reconstructing the tree of life with branch lengths comparable across all three domains. The tree has its basis in a concatenation of 31 orthologs occurring in 191 species with sequenced genomes. It revealed interdomain discrepancies in taxonomic classification. Systematic detection and subsequent exclusion of products of horizontal gene transfer increased phylogenetic resolution, allowing us to confirm accepted relationships and resolve disputed and preliminary classifications. For example, we place the phylum Acidobacteria as a sister group of delta-Proteobacteria, support a Gram-positive origin of Bacteria, and suggest a thermophilic last universal common ancestor.
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收藏
页码:1283 / 1287
页数:5
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