Stress profiling of longevity mutants identifies Afg3 as a mitochondrial determinant of cytoplasmic mRNA translation and aging

被引：61

作者：

Delaney, Joe R. ^{[1
,2
]}

Ahmed, Umema ^{[1
]}

Chou, Annie ^{[1
]}

Sim, Sylvia ^{[1
]}

Carr, Daniel ^{[1
]}

Murakami, Christopher J. ^{[1
]}

Schleit, Jennifer ^{[1
]}

Sutphin, George L. ^{[1
,2
]}

An, Elroy H. ^{[1
]}

Castanza, Anthony ^{[1
]}

Fletcher, Marissa ^{[1
]}

Higgins, Sean ^{[1
]}

Jelic, Monika ^{[1
]}

Klum, Shannon ^{[1
]}

Muller, Brian ^{[1
]}

Peng, Zhao J. ^{[1
]}

Rai, Dilreet ^{[1
]}

Ros, Vanessa ^{[1
]}

Singh, Minnie ^{[1
]}

Wende, Helen V. ^{[1
]}

Kennedy, Brian K. ^{[3
,4
]}

Kaeberlein, Matt ^{[1
,4
]}

机构：

[1] Univ Washington, Dept Pathol, Seattle, WA 98195 USA

[2] Univ Washington, Mol & Cellular Biol Program, Seattle, WA 98195 USA

[3] Buck Inst Age Res, Novato, CA USA

[4] Guangdong Med Coll, Inst Aging Res, Dongguan 523808, Peoples R China

来源：

AGING CELL | 2013年 / 12卷 / 01期

关键词：

aging; epistasis; ER stress; longevity; mitochondria; phenotype mapping; replicative lifespan; stress response; translation; yeast; CHRONOLOGICAL LIFE-SPAN; M-AAA PROTEASE; SACCHAROMYCES-CEREVISIAE; BUDDING YEAST; INNER MEMBRANE; MULTIPLE FORMS; FIBROBLASTS; RESISTANT; EXTENSION; GENES;

D O I：

10.1111/acel.12032

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Although environmental stress likely plays a significant role in promoting aging, the relationship remains poorly understood. To characterize this interaction in a more comprehensive manner, we examined the stress response profiles for 46 long-lived yeast mutant strains across four different stress conditions (oxidative, ER, DNA damage, and thermal), grouping genes based on their associated stress response profiles. Unexpectedly, cells lacking the mitochondrial AAA protease gene AFG3 clustered strongly with long-lived strains lacking cytosolic ribosomal proteins of the large subunit. Similar to these ribosomal protein mutants, afg3? cells show reduced cytoplasmic mRNA translation, enhanced resistance to tunicamycin that is independent of the ER unfolded protein response, and Sir2-independent but Gcn4-dependent lifespan extension. These data demonstrate an unexpected link between a mitochondrial protease, cytoplasmic mRNA translation, and aging.

引用

页码：156 / 166

页数：11

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