CRISPR-mediated genome editing in non-conventional yeasts for biotechnological applications

被引：106
作者：
Cai, Peng [1 ,2 ]
Gao, Jiaoqi [1 ]
Zhou, Yongjin [1 ]
机构：
[1] Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, 457 Zhongshan Rd, Dalian 116023, Peoples R China
[2] Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116023, Peoples R China
来源：
MICROBIAL CELL FACTORIES | 2019年 / 18卷 / 1期
基金：
中国国家自然科学基金;
关键词：
CRISPR-Cas9; Non-conventional yeasts; Genome editing; Guide RNA; Homologous recombination; Non-homologous end joining; METHYLOTROPHIC YEAST; HETEROLOGOUS PROTEIN; YARROWIA-LIPOLYTICA; PICHIA-PASTORIS; METABOLIC-FLUX; THERMOTOLERANT; EXPRESSION; PATHWAY; THERMOMETHANOLICA; CONSTRUCTION;
D O I：
10.1186/s12934-019-1112-2
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Non-conventional yeasts are playing important roles as cell factories for bioproduction of biofuels, food additives and proteins with outstanding natural characteristics. However, the precise genome editing is challenging in non-conventional yeasts due to lack of efficient genetic tools. In the past few years, CRISPR-based genome editing worked as a revolutionary tool for genetic engineering and showed great advantages in cellular metabolic engineering. Here, we review the current advances and barriers of CRISPR-Cas9 for genome editing in non-conventional yeasts and propose the possible solutions in enhancing its efficiency for precise genetic engineering.
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页数：12
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