Changes in soil bacterial communities following liming of acidified forests

被引:24
作者
Clivot, Hugues [1 ]
Pagnout, Christophe [1 ]
Aran, Delphine [1 ]
Devin, Simon [1 ]
Bauda, Pascale [1 ]
Poupin, Pascal [1 ]
Guerold, Francois [1 ]
机构
[1] Univ Paul Verlaine Metz, UMR CNRS 7146, Lab Interact Ecotoxicol, F-57070 Metz, France
关键词
Soil bacterial communities; Liming; Forest management; DGGE; CLPP; 16S rRNA genes; 16S RIBOSOMAL-RNA; VOSGES MOUNTAINS; MICROBIAL BIOMASS; PHYLUM ACIDOBACTERIA; ACIDIFICATION; DIVERSITY; DOMINANT; CHEMISTRY; LIBRARIES; GRADIENT;
D O I
10.1016/j.apsoil.2011.09.010
中图分类号
S15 [土壤学];
学科分类号
0903 ; 090301 ;
摘要
To counteract forest decline due to anthropogenic acidification, two catchments located on granite and on sandstone bedrock were limed (2.5 t/ha). The catchments are located in strongly affected areas in the Vosges Mountains of northeastern France. Four years after liming, structure of soil bacterial communities and community-level physiological profiling (CLPP) were investigated at two seasons. Bacterial communities were compared with those of two reference adjacent catchments to highlight changes induced by liming. Denaturing gradient gel electrophoresis (DGGE) and Biolog Ecoplate (TM) analysis revealed that liming modified the structure of bacterial communities and the CLPPs, suggesting that moderate large-scale liming has a sustainable impact on bacterial communities of forest soils. Moreover, significantly higher responses from limed soils were observed for a larger number of Biolog Ecoplate substrates compared to control soils. Analysis of 16S rRNA gene clone libraries showed that taxonomic diversity was slightly lower in limed soils, particularly within acidobacterial and Gram-positive groups, whereas proteobacterial diversity increased in limed soils. The relative abundance of dominant taxa showed that the ratio between Proteobacteria and Acidobacteria was higher in limed soils. This large-scale field study documents the effects of a four year old liming on bacterial communities of forest soils by providing a common indicator based on analysis of metabolic potential activities of heterotroph communities and by identifying major taxonomic changes in bacterial community structure. The results confirm previous observations showing that the ratio between Proteobacteria and Acidobacteria could be a microbial indicator of soil quality improvement. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:116 / 123
页数:8
相关论文
共 55 条
  • [1] Environmental chemistry - Is acidification still an ecological threat?
    Alewell, C
    Manderscheid, B
    Meesenburg, H
    Bittersohl, J
    [J]. NATURE, 2000, 407 (6806) : 856 - 857
  • [2] BASIC LOCAL ALIGNMENT SEARCH TOOL
    ALTSCHUL, SF
    GISH, W
    MILLER, W
    MYERS, EW
    LIPMAN, DJ
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) : 403 - 410
  • [3] Evaluation of streamwater composition changes in the Vosges Mountains (NE France): 1955-2005
    Angeli, N.
    Dambrine, E.
    Boudot, J. P.
    Nedeltcheva, T.
    Guerold, F.
    Tixier, G.
    Probst, A.
    Party, J. P.
    Pollier, B.
    Bourrie, G.
    [J]. SCIENCE OF THE TOTAL ENVIRONMENT, 2009, 407 (14) : 4378 - 4386
  • [4] [Anonymous], 1999, X31130 AFNOR NF, V1, P265
  • [5] [Anonymous], 1999, 10694 AFNOR NF ISO, V1, P361
  • [6] [Anonymous], 1999, 10390 AFNOR NF ISO, V1, P339
  • [7] [Anonymous], 2005, PHYLIP (phylogeny inference package) version 3.6
  • [8] BAATH E, 1980, PEDOBIOLOGIA, V20, P85
  • [9] EFFECT OF LIMING ON SOME CHEMICAL, BIOCHEMICAL, AND MICROBIOLOGICAL PROPERTIES OF ACID SOILS UNDER SPRUCE (PICEA-ABIES L)
    BADALUCCO, L
    GREGO, S
    DELLORCO, S
    NANNIPIERI, P
    [J]. BIOLOGY AND FERTILITY OF SOILS, 1992, 14 (02) : 76 - 83
  • [10] Comparison of two kinds of Biolog microplates (GN and ECO) in their ability to distinguish among aquatic microbial communities
    Choi, KH
    Dobbs, FC
    [J]. JOURNAL OF MICROBIOLOGICAL METHODS, 1999, 36 (03) : 203 - 213