Beyond sequencing: optical mapping of DNA in the age of nanotechnology and nanoscopy

被引：81

作者：

Levy-Sakin, Michal ^{[1
]}

Ebenstein, Yuval ^{[1
]}

机构：

[1] Tel Aviv Univ, Raymond & Beverly Sackler Fac Exact Sci, Sch Chem, IL-69978 Tel Aviv, Israel

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2013年 / 24卷 / 04期

基金：

以色列科学基金会;

关键词：

SINGLE-MOLECULE; GENOME SEQUENCE; ARRAYS;

D O I：

10.1016/j.copbio.2013.01.009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Next generation sequencing (NGS) is revolutionizing all fields of biological research but it fails to extract the full range of information associated with genetic material. Optical mapping of DNA grants access to genetic and epigenetic information on individual DNA molecules up to similar to 1 Mbp in length. Fluorescent labeling of specific sequence motifs, epigenetic marks and other genomic information on individual DNA molecules generates a high content optical barcode along the DNA. By stretching the DNA to a linear configuration this barcode may be directly visualized by fluorescence microscopy. We discuss the advances of these methods in light of recent developments in nano-fabrication and super-resolution optical imaging (nanoscopy) and review the latest achievements of optical mapping in the context of genomic analysis.

引用

页码：690 / 698

页数：9

共 49 条

[11] Massive Parallel Amplicon Sequencing of the Breast Cancer Genes BRCA1 and BRCA2: Opportunities, Challenges, and Limitations [J].

De Leeneer, Kim ;

Hellemans, Jan ;

De Schrijver, Joachim ;

Baetens, Machteld ;

Poppe, Bruce ;

Van Criekinge, Wim ;

De Paepe, Anne ;

Coucke, Paul ;

Claes, Kathleen .

HUMAN MUTATION, 2011, 32 (03) :335-344

[12]

Dong Y., 2012, Nat Biotechnol

[13]

Dorfman KevinD., 2012, Chemical Reviews

[14] Lighting Up Individual DNA Binding Proteins with Quantum Dots [J].

Ebenstein, Yuval ;

Gassman, Natalie ;

Kim, Soohong ;

Antelman, Josh ;

Kim, Younggyu ;

Ho, Sam ;

Samuel, Robin ;

Michalet, Xavier ;

Weiss, Shimon .

NANO LETTERS, 2009, 9 (04) :1598-1603

[15] FORUM: Genomics ENCODE explained [J].

Ecker, Joseph R. .

NATURE, 2012, 489 (7414) :52-53

[16]

Genome Reference Consortium, HUM ASS DAT

[17] Two genome sequences of the same bacterial strain, Gluconacetobacter diazotrophicus PAl 5, suggest a new standard in genome sequence submission [J].

Giongo, Adriana ;

Tyler, Heather L. ;

Zipperer, Ursula N. ;

Triplett, Eric W. .

STANDARDS IN GENOMIC SCIENCES, 2010, 2 (03) :309-317

[18] Sequence-specific covalent labelling of DNA [J].

Gottfried, Anna ;

Weinhold, Elmar .

BIOCHEMICAL SOCIETY TRANSACTIONS, 2011, 39 :623-628

[19]

Herbert S, 2012, MICROSC MICROANAL

[20]

Herrick John, 2009, V521, P71, DOI [10.1007/978-1-60327-817-5_5, 10.1007/978-1-60327-815-7_5]

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