Assessment of Antioxidant and Cytotoxicity Activities of Saponin and Crude Extracts of Chlorophytum borivilianum

被引：24

作者：

Ashraf, Mehdi Farshad ^{[1
,2
]}

Abd Aziz, Maheran ^{[1
,3
]}

Stanslas, Johnson ^{[4
]}

Ismail, Ismanizan ^{[2
]}

Kadir, Mihdzar Abdul ^{[1
]}

机构：

[1] Univ Putra Malaysia, Fac Agr, Dept Agr Technol, Serdang 43400, Selangor, Malaysia

[2] Univ Kebangsaan Malaysia, Fac Sci & Technol, Sch Biosci & Biotechnol, Bangi 43600, Selangor, Malaysia

[3] Univ Putra Malaysia, Inst Trop Agr, Lab Plantat Crops, Serdang 43400, Selangor, Malaysia

[4] Univ Putra Malaysia, Fac Med & Hlth Sci, Dept Med, Serdang 43400, Selangor, Malaysia

来源：

SCIENTIFIC WORLD JOURNAL | 2013年

关键词：

IN-VITRO ANTIOXIDANT; CAPACITY; ANALOGS; SYSTEMS; ROOTS;

D O I：

10.1155/2013/216894

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The present paper focused on antioxidant and cytotoxicity assessment of crude and total saponin fraction of Chlorophytum borivilianum as an important medicinal plant. In this study, three different antioxidant activities (2,2-diphenyl-1-picrylhydrazyl radical scavenging (DPPH), ferrous ion chelating (FIC), and beta-carotene bleaching (BCB) activity) of crude extract and total saponin fraction of C. borivilianum tubers were performed. Crude extract was found to possess higher free radical scavenging activity (ascorbic acid equivalents 2578 +/- 111mg AA/100 g) and bleaching activity (IC50 = 0.7 mg mL(-1)), while total saponin fraction displayed higher ferrous ion chelating (EC50 = 1 mg mL(-1)). Cytotoxicity evaluation of crude extract and total saponin fraction against MCF-7, PC3, and HCT-116 cancer cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) cell viability assay indicated a higher cytotoxicity activity of the crude extract than the total saponin fraction on all cell lines, being most effective and selective on MCF-7 human breast cancer cell line.

引用

页数：7

共 33 条

[1] Furostane-Type Steroidal Saponins from the Roots of Chlorophytum borivilianum [J].