Self-Assembled DNA Crystals: The Impact on Resolution of 5′-Phosphates and the DNA Source

被引：44

作者：

Sha, Ruojie ^{[1
]}

Birktoft, Jens J. ^{[1
]}

Nam Nguyen ^{[1
]}

Chandrasekaran, Arun Richard ^{[1
]}

Zheng, Jianping ^{[1
]}

Zhao, Xinshuai ^{[1
]}

Mao, Chengde ^{[2
]}

Seeman, Nadrian C. ^{[1
]}

机构：

[1] NYU, Dept Chem, New York, NY 10003 USA

[2] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

来源：

NANO LETTERS | 2013年 / 13卷 / 02期

基金：

美国国家科学基金会;

关键词：

Self-assembled 3D DNA crystals; crystal resolution; phosphorylated DNA; natural and synthetic DNA; X-ray diffraction; designed crystals;

D O I：

10.1021/nl304550c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Designed self-assembled DNA crystals consist of rigid DNA motifs that are held together by cohesive sticky-ended interactions. A prominent application of such systems is that they might be able to act as macromolecular hosts for macromolecular guests, thereby alleviating the crystallization problem of structural biology. We have recently demonstrated that it is indeed possible to design and construct such crystals and to determine their structures by X-ray diffraction procedures. To act as useful hosts that organize biological macromolecules for crystallographic purposes, maximizing the resolution of the crystals will maximize the utility of the approach. The structures reported so far have diffracted only to about 4 angstrom, so we have examined two factors that might have impact on the resolution. We find no difference in the resolution whether the DNA is synthetic or PCR-generated. However, we find that the presence of a phosphate on the 5'-end of the strands improves the resolution of the crystals markedly.

引用

页码：793 / 797

页数：5

共 11 条

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