Antitumor actions of baicalein and wogonin in HT-29 human colorectal cancer cells

被引:94
作者
Kim, So-Jung [1 ]
Kim, Hyeong-Jin [1 ]
Kim, Hye-Ri [1 ]
Lee, Seung-Ho [1 ]
Cho, Sung-Dae [2 ]
Choi, Chang-Sun [3 ]
Nam, Jeong-Seok [4 ]
Jung, Ji-Youn [1 ]
机构
[1] Kongju Natl Univ, Dept Compan & Lab Anim Sci, Yesan 340702, South Korea
[2] Chonbuk Natl Univ, Sch Dent, Dept Oral Pathol, Jeonju 561756, South Korea
[3] Chung Ang Univ, Coll Human Ecol, Dept Food & Nutr, Ansung 456756, South Korea
[4] Gachon Univ Med & Sci, Lee Gil Ya Canc & Diabet Inst, Inchon 406840, South Korea
关键词
apoptosis; baicalein; colon cancer; flavonoid; HT-29; wogonin; MEDIATED APOPTOSIS; SCUTELLARIA-BAICALENSIS; CARCINOMA CELLS; DOWN-REGULATION; RADIX; PROLIFERATION; CYTOTOXICITY; INHIBITION; FLAVONOIDS; INDUCTION;
D O I
10.3892/mmr.2012.1085
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The purpose of this study was to determine the effects of baicalein and wogonin, which are compounds derived from the Chinese herb Scutellaria baicalensis, in suppressing the viability of HT-29 human colon cancer cells. Following treatment with baicalein or wogonin, several apoptotic events were observed, including DNA fragmentation, chromatin condensation and increased cell cycle arrest in the G1 phase. Baicalein and wogonin decreased Bcl-2 expression, whereas the expression of Bax was increased in a dose-dependent manner compared with the control. Furthermore, the induction of apoptosis was accompanied by an inactivation of phosphatidylinositol 3-kinase (PI3K)/Akt in a dose-dependent manner. The administration of baicalein to mice resulted in the inhibition of the growth of HT-29 xenografts without any toxicity following 5 weeks of treatment. The results indicated that baicalein induced apoptosis via Akt activation in a p53-dependent manner in the HT-29 colon cancer cells and that it may serve as a chemopreventive or therapeutic agent for HT-29 colon cancer.
引用
收藏
页码:1443 / 1449
页数:7
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