Histomolecular interpretation of pleomorphic adenomas of the salivary gland by matrix-assisted laser desorption ionization imaging and spatial segmentation

被引：7

作者：

Ernst, Guenther ^{[1
,2
,3
]}

Guntinas-Lichius, Orlando ^{[4
]}

Hauberg-Lotte, Lena ^{[5
]}

Trede, Dennis ^{[6
]}

Becker, Michael ^{[7
]}

Alexandrov, Theodore ^{[5
,6
,8
]}

von Eggeling, Ferdinand ^{[1
,2
,3
,9
]}

机构：

[1] Univ Jena, Inst Phys Chem, Core Unit Chip Applicat, D-07745 Jena, Germany

[2] Univ Jena, Inst Human Genet, D-07745 Jena, Germany

[3] Jena Univ Hosp, Jena, Germany

[4] Jena Univ Hosp, Dept Otorhinolaryngol, Jena, Germany

[5] Univ Bremen, MALDI Imaging Lab, Dept Biol & Chem, D-28359 Bremen, Germany

[6] SCiLS GmbH, Bremen, Germany

[7] Bruker Daltonik GmbH, Bremen, Germany

[8] Univ Bremen, Ctr Ind Math, D-28359 Bremen, Germany

[9] Univ Jena, Jena Ctr Soft Matter, Jena, Germany

来源：

HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK | 2015年 / 37卷 / 07期

基金：

欧盟第七框架计划;

关键词：

pleomorphic adenoma; salivary gland; matrix-assisted laser desorption ionization (MALDI) imaging mass spectrometry; digital histology; PAROTID-GLAND; MASS-SPECTROMETRY; ESTROGEN-RECEPTOR; EXPRESSION; ORIGIN; CELLS; PROTEINS; INSULIN; CANCER; TUMOR;

D O I：

10.1002/hed.23713

中图分类号：

R76 [耳鼻咽喉科学];

学科分类号：

100213 ;

摘要：

BackgroundDespite efforts in localization of key proteins using immunohistochemistry, the complex proteomic composition of pleomorphic adenomas has not yet been characterized. Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI imaging) allows label-free and spatially resolved detection of hundreds of proteins directly from tissue sections and of histomorphological regions by finding colocalized molecular signals. Spatial segmentation of MALDI imaging data is an algorithmic method for finding regions of similar proteomic composition as functionally similar regions. MethodsWe investigated 2 pleomorphic adenomas by applying spatial segmentation to the MALDI imaging data of tissue sections. ResultsThe spatial segmentation subdivided the tissue in a good accordance with the tissue histology. Numerous molecular signals colocalized with histologically defined tissue regions were found. ConclusionOur study highlights the cellular transdifferentiation within the pleomorphic adenoma. It could be shown that spatial segmentation of MALDI imaging data is a promising approach in the emerging field of digital histological analysis and characterization of tumors. (c) 2014 Wiley Periodicals, Inc. Head Neck 37: 1014-1021, 2015

引用

页码：1014 / 1021

页数：8

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