Homogeneous assay for evaluation of aptamer-protein interaction

被引：14

作者：

Lautner, Gergely ^{[2
]}

Balogh, Zsofia ^{[3
]}

Gyurkovics, Anna ^{[3
]}

Gyurcsanyi, Robert E. ^{[1
,2
]}

Meszaros, Tamas ^{[1
,3
]}

机构：

[1] Hungarian Acad Sci, Res Grp Tech Analyt Chem, H-1111 Budapest, Hungary

[2] Budapest Univ Technol & Econ, Dept Inorgan & Analyt Chem, H-1111 Budapest, Hungary

[3] Semmelweis Univ, Dept Med Chem Mol Biol & Pathobiochem, H-1094 Budapest, Hungary

来源：

ANALYST | 2012年 / 137卷 / 17期

关键词：

IMMUNOASSAY; ALPHASCREEN;

D O I：

10.1039/c2an35419e

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We introduce Amplified Luminescent Proximity Homogenous Assay (ALPHA) to assess the K-D value of aptamer-protein complexes as demonstrated through the study of apple stem pitting virus coat protein-specific aptamers. This method can be used as a simple, cost-effective method for screening aptamer-target protein interactions during aptamer selection.

引用

页码：3929 / 3931

页数：3

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