Recombinant HCMV UL128 expression and functional identification of PBMC-attracting activity in vitro

被引：15

作者：

Gao Hui-hui ^{[1
]}

Tao Ran ^{[1
]}

Zheng Qi ^{[1
]}

Xu Jun ^{[1
]}

Shang Shi-qiang ^{[1
]}

机构：

[1] Zhejiang Univ, Sch Med, Zhejiang Key Lab Diag & Therapy Neonatal Dis, Key Lab Reprod Genet,Minist Educ,Childrens Hosp, Hangzhou 310003, Zhejiang, Peoples R China

来源：

ARCHIVES OF VIROLOGY | 2013年 / 158卷 / 01期

基金：

中国国家自然科学基金;

关键词：

HUMAN CYTOMEGALOVIRUS; ENDOTHELIAL-CELLS; CHEMOKINE; GENES; ENTRY; RECEPTORS; COMPLEX; TROPISM; SYSTEM;

D O I：

10.1007/s00705-012-1378-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Human cytomegalovirus (HCMV) has evolved several immune evasion strategies. One strategy is controlling the movement of peripheral blood mononuclear cells (PBMCs) by encoding homologues of chemokines. Our aim was to determine whether HCMV open reading frame (ORF) UL128 could encode a protein that attracts PBMCs like a beta-chemokine. The recombinant UL128 protein was synthesized by construction of a stably transfected CHO-UL128 cell line, and a chemotaxis assay showed that UL128 was able to attract PBMCs with a potency equal to that of MIP-1 alpha in vitro. We hypothesize that UL128 protein may act as a beta-chemokine homologue in viral pathogenesis.

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收藏

页码：173 / 177

页数：5

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