Integrated bioinformatics analysis of miRNA expression in Ewing sarcoma and potential regulatory effects of miR-21 via targeting ALCAM/CD166

被引：11

作者：

Liu, Yuzhe ^{[1
,2
]}

Chen, Gaoyang ^{[1
,2
,3
]}

Liu, He ^{[1
,2
]}

Li, Zhaoyan ^{[1
,2
,3
]}

Yang, Qiwei ^{[1
,3
]}

Gu, Xinming ^{[4
]}

Du, Zhenwu ^{[1
,2
,3
]}

Zhang, Guizhen ^{[1
,2
,3
]}

Wang, Jincheng ^{[1
,2
]}

机构：

[1] Jilin Univ, Hosp 2, Dept Orthopaed, Zigiang St 218, Changchun 130041, Jilin, Peoples R China

[2] Jilin Univ, Engn Res Ctr Mol Diag & Cell Treatment Metab Bone, Changchun, Jilin, Peoples R China

[3] Jilin Univ, Hosp 2, Res Ctr, Changchun, Jilin, Peoples R China

[4] Jilin Univ, Sch & Hosp Stomatol, Dept Oral Implantol, Changchun, Jilin, Peoples R China

来源：

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | 2019年 / 47卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Ewing sarcoma; miRNA expression; miR-21-3p; CD166; target genes; CELL-ADHESION MOLECULE; NCBI GEO; CANCER; TUMOR; MANAGEMENT; DATABASE; MARKER; GENE;

D O I：

10.1080/21691401.2019.1620760

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

MicroRNAs (miRNAs) play essential functions in pathogenesis of Ewing sarcoma (ES). However, the molecular mechanisms responsible for ES occurrence and development through the regulation of miRNAs remain largely unknown. This study is aimed to explore the differential expressed miRNAs and mRNAs that play vital roles in ES. GSE80201 miRNA and GSE68776 mRNA microarray dataset were selected to carry out a series of bioinformatics analysis such as GEO 2R, gene ontology, pathway enrichment analysis, Venn analysis and PPI network construction to predict hub genes. Furthermore, using quantitative real-time PCR, RNA interference and luciferase reporter assay we demonstrated that activated leukocyte cell adhesion molecule (ALCAM/CD166) is a direct target of miR-21-3p in human ES cell lines. Our results suggest that the miR-21/CD166 axis has the potential to serve as both diagnostic markers and therapeutic targets for ES.

引用

页码：2114 / 2122

页数：9

共 30 条

[11] Addition of ifosfamide and etoposide to standard chemotherapy for Ewing's sarcoma and primitive neuroectodermal tumor of bone [J].

Grier, HE ;

Krailo, MD ;

Tarbell, NJ ;

Link, MP ;

Fryer, CJH ;

Pritchard, DJ ;

Gebhardt, MC ;

Dickman, PS ;

Perlman, EJ ;

Meyers, PA ;

Donaldson, SS ;

Moore, S ;

Rausen, AR ;

Vietti, TJ ;

Miser, JS .

NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (08) :694-701

[12] ALCAM/CD166 Is a TGF-β-Responsive Marker and Functional Regulator of Prostate Cancer Metastasis to Bone [J].

Hansen, Amanda G. ;

Arnold, Shanna A. ;

Jiang, Ming ;

Palmer, Trenis D. ;

Ketova, Tatiana ;

Merkel, Alyssa ;

Pickup, Michael ;

Samaras, Susan ;

Shyr, Yu ;

Moses, Harold L. ;

Hayward, Simon W. ;

Sterling, Julie A. ;

Zijlstra, Andries .

CANCER RESEARCH, 2014, 74 (05) :1404-1415

[13] DAVID Bioinformatics Resources: expanded annotation database and novel algorithms to better extract biology from large gene lists [J].

Huang, Da Wei ;

Sherman, Brad T. ;

Tan, Qina ;

Kir, Joseph ;

Liu, David ;

Bryant, David ;

Guo, Yongjian ;

Stephens, Robert ;

Baseler, Michael W. ;

Lane, H. Clifford ;

Lempicki, Richard A. .

NUCLEIC ACIDS RESEARCH, 2007, 35 :W169-W175

[14] Increased expression of ALCAM/CD166 in pancreatic cancer is an independent prognostic marker for poor survival and early tumour relapse [J].

Kahlert, C. ;

Weber, H. ;

Mogler, C. ;

Bergmann, F. ;

Schirmacher, P. ;

Kenngott, H. G. ;

Matterne, U. ;

Mollberg, N. ;

Rahbari, N. N. ;

Hinz, U. ;

Koch, M. ;

Aigner, M. ;

Weitz, J. .

BRITISH JOURNAL OF CANCER, 2009, 101 (03) :457-464

[15] KEGG as a reference resource for gene and protein annotation [J].

Kanehisa, Minoru ;

Sato, Yoko ;

Kawashima, Masayuki ;

Furumichi, Miho ;

Tanabe, Mao .

NUCLEIC ACIDS RESEARCH, 2016, 44 (D1) :D457-D462

[16] MicroRNA expression and its clinical implications in Ewing's sarcoma [J].

Li, Zheng ;

Yu, Xin ;

Shen, Jianxiong ;

Wu, William Ka Kei ;

Chan, Matthew T. V. .

CELL PROLIFERATION, 2015, 48 (01) :1-6

[17] A novel oncogenic mechanism in Ewing sarcoma involving IGF pathway targeting by EWS/Fli1-regulated microRNAs [J].

McKinsey, E. L. ;

Parrish, J. K. ;

Irwin, A. E. ;

Niemeyer, B. F. ;

Kern, H. B. ;

Birks, D. K. ;

Jedlicka, P. .

ONCOGENE, 2011, 30 (49) :4910-4920

[18] OncomiR addiction in an in vivo model of microRNA-21-induced pre-B-cell lymphoma [J].

Medina, Pedro P. ;

Nolde, Mona ;

Slack, Frank J. .

NATURE, 2010, 467 (7311) :86-U119

[19] ALCAM (CD166): its role in hematopoietic and endothelial development [J].

Ohneda, O ;

Ohneda, K ;

Arai, F ;

Lee, J ;

Miyamoto, T ;

Fukushima, Y ;

Dowbenko, D ;

Lasky, LA ;

Suda, T .

BLOOD, 2001, 98 (07) :2134-2142

[20] FunRich: An open access standalone functional enrichment and interaction network analysis tool [J].

Pathan, Mohashin ;

Keerthikumar, Shivakumar ;

Ang, Ching-Seng ;

Gangoda, Lahiru ;

Quek, Camelia Y. J. ;

Williamson, Nicholas A. ;

Mouradov, Dmitri ;

Sieber, Oliver M. ;

Simpson, Richard J. ;

Salim, Agus ;

Bacic, Antony ;

Hill, Andrew F. ;

Stroud, David A. ;

Ryan, Michael T. ;

Agbinya, Johnson I. ;

Mariadason, John M. ;

Burgess, Antony W. ;

Mathivanan, Suresh .

PROTEOMICS, 2015, 15 (15) :2597-2601

← 1 2 3 →