Integrated bioinformatics analysis of miRNA expression in Ewing sarcoma and potential regulatory effects of miR-21 via targeting ALCAM/CD166

被引：11

作者：

Liu, Yuzhe ^{[1
,2
]}

Chen, Gaoyang ^{[1
,2
,3
]}

Liu, He ^{[1
,2
]}

Li, Zhaoyan ^{[1
,2
,3
]}

Yang, Qiwei ^{[1
,3
]}

Gu, Xinming ^{[4
]}

Du, Zhenwu ^{[1
,2
,3
]}

Zhang, Guizhen ^{[1
,2
,3
]}

Wang, Jincheng ^{[1
,2
]}

机构：

[1] Jilin Univ, Hosp 2, Dept Orthopaed, Zigiang St 218, Changchun 130041, Jilin, Peoples R China

[2] Jilin Univ, Engn Res Ctr Mol Diag & Cell Treatment Metab Bone, Changchun, Jilin, Peoples R China

[3] Jilin Univ, Hosp 2, Res Ctr, Changchun, Jilin, Peoples R China

[4] Jilin Univ, Sch & Hosp Stomatol, Dept Oral Implantol, Changchun, Jilin, Peoples R China

来源：

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | 2019年 / 47卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Ewing sarcoma; miRNA expression; miR-21-3p; CD166; target genes; CELL-ADHESION MOLECULE; NCBI GEO; CANCER; TUMOR; MANAGEMENT; DATABASE; MARKER; GENE;

D O I：

10.1080/21691401.2019.1620760

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

MicroRNAs (miRNAs) play essential functions in pathogenesis of Ewing sarcoma (ES). However, the molecular mechanisms responsible for ES occurrence and development through the regulation of miRNAs remain largely unknown. This study is aimed to explore the differential expressed miRNAs and mRNAs that play vital roles in ES. GSE80201 miRNA and GSE68776 mRNA microarray dataset were selected to carry out a series of bioinformatics analysis such as GEO 2R, gene ontology, pathway enrichment analysis, Venn analysis and PPI network construction to predict hub genes. Furthermore, using quantitative real-time PCR, RNA interference and luciferase reporter assay we demonstrated that activated leukocyte cell adhesion molecule (ALCAM/CD166) is a direct target of miR-21-3p in human ES cell lines. Our results suggest that the miR-21/CD166 axis has the potential to serve as both diagnostic markers and therapeutic targets for ES.

引用

页码：2114 / 2122

页数：9

共 30 条

[1] NCBI GEO: mining tens of millions of expression profiles - database and tools update [J].

Barrett, Tanya ;

Troup, Dennis B. ;

Wilhite, Stephen E. ;

Ledoux, Pierre ;

Rudnev, Dmitry ;

Evangelista, Carlos ;

Kim, Irene F. ;

Soboleva, Alexandra ;

Tomashevsky, Maxim ;

Edgar, Ron .

NUCLEIC ACIDS RESEARCH, 2007, 35 :D760-D765

[2] NCBI GEO: archive for functional genomics data sets-update [J].

Barrett, Tanya ;

Wilhite, Stephen E. ;

Ledoux, Pierre ;

Evangelista, Carlos ;

Kim, Irene F. ;

Tomashevsky, Maxim ;

Marshall, Kimberly A. ;

Phillippy, Katherine H. ;

Sherman, Patti M. ;

Holko, Michelle ;

Yefanov, Andrey ;

Lee, Hyeseung ;

Zhang, Naigong ;

Robertson, Cynthia L. ;

Serova, Nadezhda ;

Davis, Sean ;

Soboleva, Alexandra .

NUCLEIC ACIDS RESEARCH, 2013, 41 (D1) :D991-D995

[3] Ewing's sarcoma family of tumors: Current management [J].

Bernstein, Mark ;

Kovar, Heinrich ;

Paulussen, Michael ;

Randall, R. Lor ;

Schuck, Andreas ;

Teot, Lisa A. ;

Juergens, Herbert .

ONCOLOGIST, 2006, 11 (05) :503-519

[4] Delays in diagnosis of paediatric cancers: a systematic review and comparison with expert testimony in lawsuits [J].

Brasme, Jean-Francois ;

Morfouace, Michele ;

Grill, Jacques ;

Martinot, Alain ;

Amalberti, Rene ;

Bons-Letouzey, Catherine ;

Chalumeau, Martin .

LANCET ONCOLOGY, 2012, 13 (10) :E445-E459

[5] Ewing's sarcoma: diagnostic, and therapeutic implications of molecular abnormalities [J].

Burchill, SA .

JOURNAL OF CLINICAL PATHOLOGY, 2003, 56 (02) :96-102

[6] Expansion of the Gene Ontology knowledgebase and resources [J].

Carbon, S. ;

Dietze, H. ;

Lewis, S. E. ;

Mungall, C. J. ;

Munoz-Torres, M. C. ;

Basu, S. ;

Chisholm, R. L. ;

Dodson, R. J. ;

Fey, P. ;

Thomas, P. D. ;

Mi, H. ;

Muruganujan, A. ;

Huang, X. ;

Poudel, S. ;

Hu, J. C. ;

Aleksander, S. A. ;

McIntosh, B. K. ;

Renfro, D. P. ;

Siegele, D. A. ;

Antonazzo, G. ;

Attrill, H. ;

Brown, N. H. ;

Marygold, S. J. ;

McQuilton, P. ;

Ponting, L. ;

Millburn, G. H. ;

Rey, A. J. ;

Stefancsik, R. ;

Tweedie, S. ;

Falls, K. ;

Schroeder, A. J. ;

Courtot, M. ;

Osumi-Sutherland, D. ;

Parkinson, H. ;

Roncaglia, P. ;

Lovering, R. C. ;

Foulger, R. E. ;

Huntley, R. P. ;

Denny, P. ;

Campbell, N. H. ;

Kramarz, B. ;

Patel, S. ;

Buxton, J. L. ;

Umrao, Z. ;

Deng, A. T. ;

Alrohaif, H. ;

Mitchell, K. ;

Ratnaraj, F. ;

Omer, W. ;

Rodriguez-Lopez, M. .

NUCLEIC ACIDS RESEARCH, 2017, 45 (D1) :D331-D338

[7] A TARBP2-Dependent miRNA Expression Profile Underlies Cancer Stem Cell Properties and Provides Candlidate Therapeutic Reagents in Ewing Sarcoma [J].

De Vito, Claudio ;

Riggi, Nicolo ;

Cornaz, Sandrine ;

Suva, Mario-Luca ;

Baumer, Karine ;

Provero, Paolo ;

Stamenkovic, Ivan .

CANCER CELL, 2012, 21 (06) :807-821

[8] Using microRNA Networks to Understand Cancer [J].

Dragomir, Mihnea ;

Mafra, Ana Carolina P. ;

Dias, Sandra M. G. ;

Vasilescu, Catalin ;

Calin, George A. .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2018, 19 (07)

[9] Focal adhesion signaling and therapy resistance in cancer [J].

Eke, Iris ;

Cordes, Nils .

SEMINARS IN CANCER BIOLOGY, 2015, 31 :65-75

[10] Ewing Sarcoma: Current Management and Future Approaches Through Collaboration [J].

Gaspar, Nathalie ;

Hawkins, Douglas S. ;

Dirksen, Uta ;

Lewis, Ian J. ;

Ferrari, Stefano ;

Le Deley, Marie-Cecile ;

Kovar, Heinrich ;

Grimer, Robert ;

Whelan, Jeremy ;

Claude, Line ;

Delattre, Olivier ;

Paulussen, Michael ;

Picci, Piero ;

Hall, Kirsten Sundby ;

van den Berg, Hendrik ;

Ladenstein, Ruth ;

Michon, Jean ;

Hjorth, Lars ;

Judson, Ian ;

Luksch, Roberto ;

Bernstein, Mark L. ;

Marec-Berard, Perrine ;

Brennan, Bernadette ;

Craft, Alan W. ;

Womer, Richard B. ;

Juergens, Heribert ;

Oberlin, Odile .

JOURNAL OF CLINICAL ONCOLOGY, 2015, 33 (27) :3036-U140

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