A microfluidic device for the hydrodynamic immobilisation of living fission yeast cells for super-resolution imaging

被引：32
|
作者：
Bell, Laurence [1 ]
Seshia, Ashwin [1 ]
Lando, David [2 ]
Laue, Ernest [2 ]
Palayret, Matthieu [3 ]
Lee, Steven F. [3 ]
Klenerman, David [3 ]
机构：
[1] Cambridge Nanosci Ctr, Cambridge CB3 0FF, England
[2] Dept Biochem, Cambridge CB2 1GA, England
[3] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2014年 / 192卷
基金：
英国惠康基金;
关键词：
Microfluidics; Single cell analysis; Hydrodynamic trapping; PALM imaging; Single molecule localisation microscopy; CAULOBACTER-CRESCENTUS; LOCALIZATION; MICROSCOPY;
D O I：
10.1016/j.snb.2013.10.002
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We describe a microfluidic device designed specifically for the reversible immobilisation of Schizosaccharomyces pombe (Fission Yeast) cells to facilitate live cell super-resolution microscopy. Photo-Activation Localisation Microscopy (PALM) is used to create detailed super-resolution images within living cells with a modal accuracy of >25 nm in the lateral dimensions. The novel flow design captures and holds cells in a well-defined array with minimal effect on the normal growth kinetics. Cells are held over several hours and can continue to grow and divide within the device during fluorescence imaging. (C) 2013 The Authors. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：36 / 41
页数：6
相关论文
共 50 条
  • [31] Super-resolution Raman probe imaging
    Ozeki, Yasuyuki
    Shou, Jingwen
    Komazawa, Ayumi
    Kamiya, Mako
    ADVANCED CHEMICAL MICROSCOPY FOR LIFE SCIENCE AND TRANSLATIONAL MEDICINE 2024, 2024, 12855
  • [32] Super-resolution imaging within reach
    Perrin, Stephane
    Montgomery, Paul
    Lecler, Sylvain
    OPTICAL ENGINEERING, 2019, 58 (05)
  • [33] Super-Resolution Imaging with Patchy Microspheres
    Shang, Qingqing
    Tang, Fen
    Yu, Lingya
    Oubaha, Hamid
    Caina, Darwin
    Yang, Songlin
    Melinte, Sorin
    Zuo, Chao
    Wang, Zengbo
    Ye, Ran
    PHOTONICS, 2021, 8 (11)
  • [34] A molecular probe based on pyrimidine imidazole derivatives for stable super-resolution endoplasmic reticulum imaging in living cells
    Hu, Ling
    Hussain, Sajid
    Liu, Tianyan
    Yue, Yuanzhen
    Liu, Jiejie
    Tian, Yupeng
    Tian, Xiaohe
    NEW JOURNAL OF CHEMISTRY, 2018, 42 (18) : 14725 - 14728
  • [35] Multicolor re-scan super-resolution imaging of live cells
    Liao, Jiuling
    Chen, Longchao
    Xia, Xianyuan
    Yu, Jia
    Chen, Tingai
    Li, Hui
    Zheng, Wei
    QUANTITATIVE IMAGING IN MEDICINE AND SURGERY, 2019, 9 (05) : 815 - 822
  • [36] Deep learning for fast super-resolution ultrasound microvessel imaging
    Luan, Shunyao
    Yu, Xiangyang
    Lei, Shuang
    Ma, Chi
    Wang, Xiao
    Xue, Xudong
    Ding, Yi
    Ma, Teng
    Zhu, Benpeng
    PHYSICS IN MEDICINE AND BIOLOGY, 2023, 68 (24)
  • [37] Aptamer AS1411 utilized for super-resolution imaging of nucleolin
    Jing, Yingying
    Cai, Mingjun
    Zhou, Lulu
    Jiang, Junguang
    Gao, Jing
    Wang, Hongda
    TALANTA, 2020, 217
  • [38] 3D Super-Resolution Fluorescence Imaging of Microgels
    Nevskyi, Oleksii
    Woell, Dominik
    ANNUAL REVIEW OF PHYSICAL CHEMISTRY, 2023, 74 : 391 - 414
  • [39] Single molecule super-resolution imaging of proteins in living Salmonella enterica using self-labelling enzymes
    Barlag, Britta
    Beutel, Oliver
    Janning, Dennis
    Czarniak, Frederik
    Richter, Christian P.
    Kommnick, Carina
    Goeser, Vera
    Kurre, Rainer
    Fabiani, Florian
    Erhardt, Marc
    Piehler, Jacob
    Hensel, Michael
    SCIENTIFIC REPORTS, 2016, 6
  • [40] A neural lens for super-resolution biological imaging
    Grant-Jacob, James A.
    Mackay, Benita S.
    Baker, James A. G.
    Xie, Yunhui
    Heath, Daniel J.
    Loxham, Matthew
    Eason, Robert W.
    Mills, Ben
    JOURNAL OF PHYSICS COMMUNICATIONS, 2019, 3 (06):
12345