Identification of tetratricopeptide repeat domain 9, a hormonally regulated protein

被引：19

作者：

Cao, Shenglan ^{[1
]}

Iyer, Jayasree K. ^{[1
]}

Lin, Valerie ^{[1
]}

机构：

[1] Nanyang Technol Univ, Sch Biol Sci, Singapore, Singapore

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 345卷 / 01期

关键词：

tetratricopeptide repeat domain 9; breast cancer; endoplasmic reticulum; growth factors; steroid hormones;

D O I：

10.1016/j.bbrc.2006.04.091

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tetratricopeptide repeat domain 9 (TTC9) mRNA was drastically up-regulated by progesterone in progesterone receptor-transfected breast cancer cells MDA-MB-231. This up-regulation is coupled with progesterone-mediated growth inhibition and induction of focal adhesion. We have generated mouse polyclonal antibody against a predicted 222 aa TTC9 protein and identified a 25 kDa TTC9 protein that is widely expressed in human tissues, with the highest expression in the brain. Immunostaining and cell fractionation studies revealed that TTC9 is predominantly localized to the endoplasmic reticulum. The level of TTC9 protein in MCF-7 cells is regulated by various factors and chemical reagents including estrogen, progesterone, growth factors, ICI182,780, and p38 kinase inhibitor SB203580. Growth factor-induced TTC9 protein expression was inhibited by estrogen and abolished by ERK inhibitor PD98059. Though the function of TTC9 is not yet clear, the susceptibility of its protein level to biological and chemical agents suggests that TTC9 is a biologically significant protein. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：310 / 317

页数：8

共 18 条

[1] Selective estrogen-receptor modulators for primary prevention of breast cancer [J].

Fabian, CJ ;

Kimler, BF .

JOURNAL OF CLINICAL ONCOLOGY, 2005, 23 (08) :1644-1655

[2] The biology and enzymology of protein N-myristoylation [J].

Farazi, TA ;

Waksman, G ;

Gordon, JI .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (43) :39501-39504

[3] Host microenvironment in breast cancer development - Epithelial-cell-stromal-cell interactions and steroid hormone action in normal and cancerous mammary gland [J].

Haslam, SZ ;

Woodward, TL .

BREAST CANCER RESEARCH, 2003, 5 (04) :208-215

[4] A complex between peptide:N-glycanase and two proteasome-linked proteins suggests a mechanism for the degradation of misfolded glycoproteins [J].

Katiyar, S ;

Li, GT ;

Lennarz, WJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (38) :13774-13779

[5] A SIMPLE METHOD FOR DISPLAYING THE HYDROPATHIC CHARACTER OF A PROTEIN [J].

KYTE, J ;

DOOLITTLE, RF .

JOURNAL OF MOLECULAR BIOLOGY, 1982, 157 (01) :105-132

[6] Glucocorticoid and mineralocorticoid cross-talk with progesterone receptor to induce focal adhesion and growth inhibition in breast cancer cells [J].

Leo, JCL ;

Guo, CH ;

Woon, CT ;

Aw, SE ;

Lin, VCL .

ENDOCRINOLOGY, 2004, 145 (03) :1314-1321

[7]

Lin VCL, 2001, CLIN CANCER RES, V7, P2880

[8] Progesterone induces focal adhesion in breast cancer cells MDA-MB-231 transfected with progesterone receptor complementary DNA [J].

Lin, VCL ;

Ng, EH ;

Aw, SE ;

Tan, MGK ;

Ng, EHL ;

Bay, BH .

MOLECULAR ENDOCRINOLOGY, 2000, 14 (03) :348-358

[9]

Lin VCL, 1999, CLIN CANCER RES, V5, P395

[10] Tyrosine kinase/p21(ras)/MAP-kinase pathway activation by estradiol-receptor complex in MCF-7 cells [J].

Migliaccio, A ;

DiDomenico, M ;

Castoria, G ;

deFalco, A ;

Bontempo, P ;

Nola, E ;

Auricchio, F .

EMBO JOURNAL, 1996, 15 (06) :1292-1300

← 1 2 →