An engineered protease that cleaves specifically after sulfated tyrosine

被引：24

作者：

Varadarajan, Navin ^{[1
,2
]}

Georgiou, George ^{[1
,3
]}

Iverson, Brent L. ^{[2
]}

机构：

[1] Univ Texas Austin, Dept Chem Engn, Austin, TX 78712 USA

[2] Univ Texas Austin, Dept Chem & Biochem, Austin, TX 78712 USA

[3] Univ Texas Austin, Inst Cell & Mol Biol, Austin, TX 78712 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 41期

基金：

美国国家卫生研究院;

关键词：

post-translational modification; proteases; protein engineering;

D O I：

10.1002/anie.200800736

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Picky eater: The bacterial protease OmpT has been engineered to cleave after sulfotyrosine residues in peptide substrates but not after phosphotyrosine (see scheme). A selection/counterselection screen was used to identify OmpTvariants with the desired specificity and high levels of overall catalytic activity. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：7861 / 7863

页数：3

共 23 条

[21] ADAM17 is the main sheddase for the generation of human triggering receptor expressed in myeloid cells (hTREM2) ectodomain and cleaves TREM2 after Histidine 157
Feuerbach, Dominik
Schindler, Patrick
Barske, Carmen
Joller, Stefanie
Beng-Louka, Edwige
Worringer, Katie A.
Kommineni, Sravya
Kaykas, Ajamete
Ho, Daniel J.
Ye, Chaoyang
Welzenbach, Karl
Elain, Gaelle
Klein, Laurent
Brzak, Irena
Mir, Anis K.
Farady, Christopher J.
Aichholz, Reiner
Popp, Simone
George, Nathalie
Neumann, Ulf
NEUROSCIENCE LETTERS, 2017, 660 : 109 - 114
[22] Crystal structures of apo wild-type M-jannaschii tyrosyl-tRNA synthetase (TyrRS) and an engineered TyrRS specific for O-methyl-L-tyrosine
Zhang, Y
Wang, L
Schultz, PG
Wilson, IA
PROTEIN SCIENCE, 2005, 14 (05) : 1340 - 1349
[23] Structure of the NS2B-NS3 protease from Zika virus after self-cleavage
Phoo, Wint Wint
Li, Yan
Zhang, Zhenzhen
Lee, Michelle Yueqi
Loh, Ying Ru
Tan, Yaw Bia
Ng, Elizabeth Yihui
Lescar, Julien
Kang, CongBao
Luo, Dahai
NATURE COMMUNICATIONS, 2016, 7

← 1 2 3 →