Mature IgM-expressing plasma cells sense antigen and develop competence for cytokine production upon antigenic challenge

被引：68

作者：

Blanc, Pascal ^{[1
]}

Moro-Sibilot, Ludovic ^{[1
]}

Barthly, Lucas ^{[1
]}

Jagot, Ferdinand ^{[1
]}

This, Sebastien ^{[1
]}

de Bernard, Simon ^{[2
]}

Buffat, Laurent ^{[2
]}

Dussurgey, Sebastien ^{[3
]}

Colisson, Renaud ^{[4
]}

Hobeika, Elias ^{[5
]}

Fest, Thierry ^{[6
,7
,8
]}

Taillardet, Morgan ^{[1
]}

Thaunat, Olivier ^{[1
]}

Sicard, Antoine ^{[1
]}

Mondiere, Paul ^{[1
]}

Genestier, Laurent ^{[1
]}

Nutt, Stephen L. ^{[9
,10
]}

Defrance, Thierry ^{[1
]}

机构：

[1] Univ Claude Bernard Lyon 1, Ecole Normale Super Lyon, Univ Lyon, CIRI,INSERM,U1111,CNRS,UMR5308, 21 Ave Tony Garnier, F-69007 Lyon, France

[2] AltraBio SAS, 30 Rue Pre Gaudry, F-69007 Lyon, France

[3] INSERM SFR Biosci Gerland, UMS3444, US8, 50 Ave Tony Garnier, F-69007 Lyon, France

[4] eBioscience, 140 Bis Rue Rennes, F-75006 Paris, France

[5] Univ Hosp, Inst Immunol, Albert Einstein Allee 11, D-89073 Ulm, Germany

[6] INSERM, UMR917, F-35043 Rennes, France

[7] Ctr Hosp Univ, Pole Biol, F-35033 Rennes, France

[8] Univ Rennes 1, F-35065 Rennes, France

[9] Walter & Eliza Hall Inst Med Res, 1G Royal Parade, Parkville, Vic 3052, Australia

[10] Univ Melbourne, Dept Med Biol, Parkville, Vic 3010, Australia

来源：

NATURE COMMUNICATIONS | 2016年 / 7卷

基金：

英国医学研究理事会;

关键词：

B-CELLS; IMMUNOGLOBULIN; BLIMP-1; GENE; RESPONSES; IMMUNITY; MEMORY; DIFFERENTIATION; PATHWAYS; SURVIVAL;

D O I：

10.1038/ncomms13600

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Dogma holds that plasma cells, as opposed to B cells, cannot bind antigen because they have switched from expression of membrane-bound immunoglobulins (Ig) that constitute the B-cell receptor (BCR) to production of the secreted form of immunoglobulins. Here we compare the phenotypical and functional attributes of plasma cells generated by the T-cell-dependent and T-cell-independent forms of the hapten NP. We show that the nature of the secreted Ig isotype, rather than the chemical structure of the immunizing antigen, defines two functionally distinct populations of plasma cells. Fully mature IgM-expressing plasma cells resident in the bone marrow retain expression of a functional BCR, whereas their IgG(+) counterparts do not. Antigen boost modifies the gene expression profile of IgM(+) plasma cells and initiates a cytokine production program, characterized by upregulation of CCL5 and IL-10. Our results demonstrate that IgM-expressing plasma cells can sense antigen and acquire competence for cytokine production upon antigenic challenge.

引用

页数：14

共 52 条

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