Simultaneous separation of anions and cations by flow-gradient ion chromatography

被引:9
|
作者
Deguchi, K
Kohda, K
Ito, M
机构
[1] Hitachi Instrument Engn Co Ltd, Techno Res Ctr, Ibaraki 3128504, Japan
[2] Hitachi Ltd, Hitachi Instrument Div, Customer Support Ctr, Ibaraki 3128504, Japan
关键词
flow-gradient chromatography; gradient elution; inorganic anions; inorganic cations;
D O I
10.1016/S0021-9673(99)00157-0
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A new method to allow the simultaneous separation of anions and cations is proposed. It uses both the anion- and cation-exchange column connected in series, and uses two pumps with a flow-gradient capability. Two eluents delivered by these two pumps are merged in between the two columns. By varying me flow rates of two pumps and keeping the total flow-rate constant, retention times of both anions and cations can be controlled so that they are well separated. This new method was applied to a simultaneous separation of monovalent cations and anions. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:165 / 170
页数:6
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