Successful bacterial incorporation into activated sludge flocs using alginate

被引:36
作者
Bouchez, T. [1 ,2 ]
Patureau, D. [2 ]
Delgenes, J. P. [2 ]
Moletta, R. [2 ]
机构
[1] Cemagref, UR HBAN, F-92163 Antony, France
[2] Inst Natl Rech Agron, Lab Biotechnol Environm, F-11100 Narbonne, France
关键词
Bioaugmentation; Alginate; Microvirgula aerodenitrificans; Aerobic denitrification; Fluorescent in situ hybridization; BIOLOGICAL PHOSPHORUS REMOVAL; WASTE-WATER; BIOAUGMENTATION; HYBRIDIZATION; INOCULUM; PROBES;
D O I
10.1016/j.biortech.2008.07.028
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Bioaugmentation experiments with the aerobic denitrifier Microvirgula aerodenitrificans were performed in an aerobic continuous stirred tank reactor (CSTR) treating urban wastewater. The fate of the added bacteria was monitored by a specific fluorescent oligonucleotide probe targeting 16S rRNA. The first addition of the strain led to its rapid disappearance because of grazing. Bacteria were then embedded within an alginate matrix before inoculation. Alginate fragments adhered to the existing flocs and were progressively colonized by the indigenous flora. Thereafter, microcolonies of the exogenous bacterium were found to be incorporated into existing flocs. (c) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1031 / 1032
页数:2
相关论文
共 18 条
[2]   Plasmid-mediated bioaugmentation of activated sludge bacteria in a sequencing batch moving bed reactor using pNB2 [J].
Bathe, S ;
Schwarzenbeck, N ;
Hausner, M .
LETTERS IN APPLIED MICROBIOLOGY, 2005, 41 (03) :242-247
[3]   The bioaugmentation of sequencing batch reactor sludges for biological phosphorus removal [J].
Belia, E ;
Smith, PG .
WATER SCIENCE AND TECHNOLOGY, 1997, 35 (01) :19-26
[4]   Triggers for microbial aggregation in activated sludge? [J].
Bossier, P ;
Verstraete, W .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1996, 45 (1-2) :1-6
[5]  
Bouchez T, 2000, ENVIRON MICROBIOL, V2, P179
[6]   Successful and unsuccessful bioaugmentation experiments monitored by fluorescent in situ hybridization [J].
Bouchez, T ;
Patureau, D ;
Dabert, P ;
Wagner, M ;
Delgenès, JP ;
Moletta, R .
WATER SCIENCE AND TECHNOLOGY, 2000, 41 (12) :61-68
[7]   Bioaugmentation of soils by increasing microbial richness: missing links [J].
Dejonghe, W ;
Boon, N ;
Seghers, D ;
Top, EM ;
Verstraete, W .
ENVIRONMENTAL MICROBIOLOGY, 2001, 3 (10) :649-657
[8]   Is bioaugmentation a feasible strategy for pollutant removal and site remediation? [J].
El Fantroussi, S ;
Agathos, SN .
CURRENT OPINION IN MICROBIOLOGY, 2005, 8 (03) :268-275
[9]   Microstructure of anaerobic granules bioaugmented with Desulfitobacterium frappieri PCP-1 [J].
Lanthier, M ;
Tartakovsky, B ;
Villemur, R ;
DeLuca, G ;
Guiot, SR .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (08) :4035-4043
[10]   Performance of a commercial inoculum for the aerobic biodegradation of a high fat content dairy wastewater [J].
Loperena, Lyliam ;
Ferrari, Mario Daniel ;
Saravia, Veronica ;
Murro, Daiman ;
Lima, Cynthia ;
Ferrando, Lucia ;
Fernandez, Ana ;
Lareo, Claudia .
BIORESOURCE TECHNOLOGY, 2007, 98 (05) :1045-1051