Stability of plasmid and viral banks supporting the cGMP manufacture of Q-Griffithsin from a TMV-based viral vector

被引：5

作者：

Corman, Julia M. ^{[2
]}

Hamorsky, Krystal T. ^{[1
,2
,3
]}

Shepherd, John W. ^{[6
]}

Hiatt, Ernie ^{[5
]}

Fuqua, Joshua L. ^{[1
,2
,4
]}

Palmer, Kenneth E. ^{[1
,2
,4
]}

机构：

[1] Univ Louisville, Sch Med, James Graham Brown Canc Ctr, Louisville, KY 40292 USA

[2] Univ Louisville, Sch Med, Ctr Predict Med, Louisville, KY 40292 USA

[3] Univ Louisville, Sch Med, Dept Med, Louisville, KY 40292 USA

[4] Univ Louisville, Sch Med, Dept Pharmacol & Toxicol, Louisville, KY 40292 USA

[5] RJ Reynolds Tobacco Co, Winston Salem, NC USA

[6] Kentucky Bioproc Inc, Owensboro, KY USA

来源：

JOURNAL OF BIOTECHNOLOGY | 2020年 / 320卷

关键词：

Q-Griffithsin; Microbicide; TMV; Plant-made pharmaceuticals; Virion; Stability; EXPRESSION;

D O I：

10.1016/j.jbiotec.2020.06.009

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The "whole genome" TMV-based expression system, Geneware (R), was used in the cGMP production of the plant-made pharmaceutical Q-Griffithsin and demonstrates stable expression for up to a two-year period. Virion and plasmid banks which contained viral cDNA and a Q-Griffithsin sequence were able to produce > 200 g of Q-Griffithsin. Data assessing the quality and stability of the product banks were measured through functional assessments of visual symptomology and product expression.

引用

页码：74 / 76

页数：3

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