Programmable Magnetic Tweezers and Droplet Microfluidic Device for High-Throughput Nanoliter Multi-Step Assays

被引：65

作者：

Ali-Cherif, Anais ^{[1
]}

Begolo, Stefano ^{[1
]}

Descroix, Stephanie ^{[1
]}

Viovy, Jean-Louis ^{[1
]}

Malaquin, Laurent ^{[1
]}

机构：

[1] CNRS, Res Ctr, Inst Curie UMR 168, UMR168, F-75005 Paris, France

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 43期

关键词：

droplets; fluorescence; immunoassays; magnetic particles; microfluidics; PLATFORM; ENCAPSULATION; MANIPULATION; CELLS;

D O I：

10.1002/anie.201203862

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Tweezing out the answer: A microfluidic device combining droplets (less than 100 nL) and magnetic particles (see scheme) was implemented for fast heterogeneous multiplexed assays. Magnetic tweezers can perform the manipulations required in an immunoassay (capture, extraction, mixing, and rinsing). This method was applied to the diagnosis of congenital hypothyroidism with 14 pM sensitivity. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：10765 / 10769

页数：5

共 32 条

[1] Ultrahigh-throughput screening in drop-based microfluidics for directed evolution [J].

Agresti, Jeremy J. ;

Antipov, Eugene ;

Abate, Adam R. ;

Ahn, Keunho ;

Rowat, Amy C. ;

Baret, Jean-Christophe ;

Marquez, Manuel ;

Klibanov, Alexander M. ;

Griffiths, Andrew D. ;

Weitz, David A. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (09) :4004-4009

[2]

Alhetlani E., 2010, MUTAS P, P1817

[3]

[Anonymous], 2006, ANGEW CHEM INT ED, V45, P3062

[4]

[Anonymous], 2010, ANGEW CHEM INT ED, V49, P5846

[5]

[Anonymous], 2006, ANGEW CHEM INT ED, V45, P7336

[6]

[Anonymous], 2003, ANGEW CHEM INT ED, V42, P768

[7] Dynamics of microfluidic droplets [J].

Baroud, Charles N. ;

Gallaire, Francois ;

Dangla, Remi .

LAB ON A CHIP, 2010, 10 (16) :2032-2045

[8]

Begolo S, 2011, LAB CHIP, V11, P508, DOI [10.1039/c0lc00356e, 10.1039/c01c00356e]

[9] Streamlining Immunoassays with Immiscible Filtrations Assisted by Surface Tension [J].

Berry, Scott M. ;

Maccoux, Lindsey J. ;

Beebe, David J. .

ANALYTICAL CHEMISTRY, 2012, 84 (13) :5518-5523

[10] Droplet fusion by alternating current (AC) field electrocoalescence in microchannels [J].

Chabert, M ;

Dorfman, KD ;

Viovy, JL .

ELECTROPHORESIS, 2005, 26 (19) :3706-3715

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