A potentiometric biosensor for D-amygdalin based on a consolidated biocomposite membrane

A new potentiometric biosensor for D-amygdalin based on a consolidated biocomposite membrane has been designed. The manufacturing technique involved a simple compression of dry components presenting an ideal technology for the mass production of potentiometric biosensor devices. The effect of high pressures applied during the preparation of a D-amygdalin biosensor membrane was studied. The immobilisation of beta-glucosidase was improved by increasing this pressure. The optimal biocomposite membrane composition was 100 mg electroactive salts (AgI, Ag2S) and 10 mg enzyme (50 U). Characteristics such as linear range, response times and degree of enzyme immobilisation were studied for each of the pressures used in the production of the membranes. (C) 1999 Elsevier Science B.V. All rights reserved.