High yield and optical purity in biocatalysed acylation of trans-2-phenyl-1-cyclohexanol with Candida rugosa lipase in non-conventional media

被引：16

作者：

Celia, EC

Cernia, E

D'Acquarica, I

Palocci, C

Soro, S

机构：

[1] Univ Rome La Sapienza, Dipartimento Chim, I-00185 Rome, Italy

[2] Univ Rome La Sapienza, Dipartmento Chim & Tecnol Sostanze Biologicamente, I-00185 Rome, Italy

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 1999年 / 6卷 / 05期

关键词：

lipase; transesterification; trans-2-phenyl-1-cyclohexanol; resolution; organic media; supercritical fluids;

D O I：

10.1016/S1381-1177(99)00014-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Candida rugosa lipase (CRL) shows high enantioselectivity toward (1R,2S)-(-)-trans-2-phenyl-1-cyclohexanol enantiomer in acetylation reaction employing vinyl acetate as acyl donor. Attempts to improve reaction yields have pointed out that supercritical CO2 is the best reaction medium in the studied biocatalytic process. In these conditions an immobilised lipase from Candida rugosa is able to quantitatively resolve: racemate with e.e.(p) 100%. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：495 / 503

页数：9

共 31 条

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