Extracellular Vesicles in Diagnosis and Treatment of Pancreatic Cancer: Current State and Future Perspectives

被引：17

作者：

Lane, J. Spencer ^{[1
]}

Von Hoff, Daniel ^{[2
]}

Cridebring, Derek ^{[2
]}

Goel, Ajay ^{[3
]}

机构：

[1] Univ Texas Houston, Internal Med Pediat, Houston, TX 77030 USA

[2] Translat Genom Res Inst, TGen, Phoenix, AZ 85004 USA

[3] City Hope Natl Med Ctr, Beckman Res Inst, Dept Mol Diagnost & Expt Therapeut, Duarte, CA 91010 USA

来源：

CANCERS | 2020年 / 12卷 / 06期

关键词：

pancreatic cancer; exosomes; extracellular vesicles; early detection; biomarkers; BIOLOGICAL FUNCTIONS; MASS-SPECTROMETRY; EXOSOMES; CELLS; IDENTIFICATION; EXPRESSION; MICRORNAS; APOPTOSIS; PROGRESS; CA19-9;

D O I：

10.3390/cancers12061530

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Pancreatic cancer remains one of the deadliest diagnoses a patient can receive. One of the reasons for this lethality is that this malignancy is often detected very late due to a lack of symptoms during the early stages. In addition to the lack of symptoms, we currently do not have a reliable biomarker for screening. Carbohydrate antigen (CA) 19-9 has a sensitivity between 79% and 84% and a specificity of 82-90%, making it unreliable for early detection. Recently, there have been numerous studies on the use of extracellular vesicles (EVs) to detect pancreas cancer. This field has been rapidly expanding, with new methods and biomarkers being introduced regularly. This review provides a systematic update on the commonly used and promising methods used in the detection of EVs, biomarkers associated with EVs for early detection and prognosis, as well as studies looking at using EVs as therapeutics. The review ends with remarks about areas to focus on using EVs going forward.

引用

页码：1 / 18

页数：17

共 79 条

[31] Insights into the Role of microRNAs in Pancreatic Cancer Pathogenesis: Potential for Diagnosis, Prognosis, and Therapy [J].

Khan, Mohammad Aslam ;

Zubair, Haseeb ;

Srivastava, Sanjeev Kumar ;

Singh, Seema ;

Singh, Ajay Pratap .

MICRORNA: CANCER: FROM MOLECULAR BIOLOGY TO CLINICAL PRACTICE, 2015, 889 :71-87

[32] Isolation of Extracellular Vesicles: General Methodologies and Latest Trends [J].

Konoshenko, Maria Yu. ;

Lekchnov, Evgeniy A. ;

Vlassov, Alexander V. ;

Laktionov, Pavel P. .

BIOMED RESEARCH INTERNATIONAL, 2018, 2018

[33] Exosomes: Vehicles of Intercellular Signaling, Biomarkers, and Vectors of Cell Therapy [J].

Kourembanas, Stella .

ANNUAL REVIEW OF PHYSIOLOGY, VOL 77, 2015, 77 :13-27

[34] Cancer-associated fibroblast-derived annexin A6+ extracellular vesicles support pancreatic cancer aggressiveness [J].

Leca, Julie ;

Martinez, Sebastien ;

Lac, Sophie ;

Nigri, Jeremy ;

Secq, Veronique ;

Rubis, Marion ;

Bressy, Christian ;

Serge, Arnauld ;

Lavaut, Marie-Noelle ;

Dusetti, Nelson ;

Loncle, Celine ;

Rogues, Julie ;

Pietrasz, Daniel ;

Bousquet, Corinne ;

Garcia, Stephane ;

Granjeaud, Samuel ;

Ouaissi, Mehdi ;

Bachet, Jean Baptiste ;

Brun, Christine ;

Iovanna, Juan L. ;

Zimmermann, Pascale ;

Vasseur, Sophie ;

Tomasini, Richard .

JOURNAL OF CLINICAL INVESTIGATION, 2016, 126 (11) :4140-4156

[35] Progress in Exosome Isolation Techniques [J].

Li, Pin ;

Kaslan, Melisa ;

Lee, Sze Han ;

Yao, Justin ;

Gao, Zhiqiang .

THERANOSTICS, 2017, 7 (03) :789-804

[36] Nanoplasmonic quantification of tumour-derived extracellular vesicles in plasma microsamples for diagnosis and treatment monitoring [J].

Liang, Kai ;

Liu, Fei ;

Fan, Jia ;

Sun, Dali ;

Liu, Chang ;

Lyon, Christopher J. ;

Bernard, David W. ;

Li, Yan ;

Yokoi, Kenji ;

Katz, Matthew H. ;

Koay, Eugene J. ;

Zhao, Zhen ;

Hu, Ye .

NATURE BIOMEDICAL ENGINEERING, 2017, 1 (04)

[37] Optimized exosome isolation protocol for cell culture supernatant and human plasma [J].

Lobb, Richard J. ;

Becker, Melanie ;

Wen, Shu Wen ;

Wong, Christina S. F. ;

Wiegmans, Adrian P. ;

Leimgruber, Antoine ;

Moeller, Andreas .

JOURNAL OF EXTRACELLULAR VESICLES, 2015, 4

[38] Precipitation with polyethylene glycol followed by washing and pelleting by ultracentrifugation enriches extracellular vesicles from tissue culture supernatants in small and large scales [J].

Ludwig, Anna-Kristin ;

De Miroschedji, Kyra ;

Doeppner, Thorsten R. ;

Boerger, Verena ;

Ruesing, Johannes ;

Rebmann, Vera ;

Durst, Stephan ;

Jansen, Soeren ;

Bremer, Michel ;

Behrmann, Elmar ;

Singer, Bernhard B. ;

Jastrow, Holger ;

Kuhlmann, Jan Dominik ;

El Magraoui, Fouzi ;

Meyer, Helmut E. ;

Hermann, Dirk M. ;

Opalka, Bertram ;

Raunser, Stefan ;

Epple, Matthias ;

Horn, Peter A. ;

Giebel, Bernd .

JOURNAL OF EXTRACELLULAR VESICLES, 2018, 7 (01)

[39]

Luna AS, 2017, ENCYCLOPEDIA OF SPECTROSCOPY AND SPECTROMETRY, 3RD EDITION, VOL 3: N-R, P919, DOI 10.1016/B978-0-12-409547-2.12171-7

[40] High-Performance Chemical Isotope Labeling Liquid Chromatography Mass Spectrometry for Exosome Metabolomics [J].

Luo, Xian ;

An, Mingrui ;

Cuneo, Kyle C. ;

Lubman, David M. ;

Li, Liang .

ANALYTICAL CHEMISTRY, 2018, 90 (14) :8314-8319

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