Effect of Short-Term Administration of Glucagon on Gene Expression of the Insulin Receptor in Primary Cultured Calf Hepatocytes

被引：0

作者：

Zhang, Z. G. ^{[1
]}

Li, X. B. ^{[2
]}

Liu, G. W. ^{[2
]}

Chen, Y. Y. ^{[1
]}

Yu, M. L. ^{[1
]}

Wang, J. G. ^{[2
]}

Wang, H. B. ^{[1
]}

Liu, L. ^{[1
,2
]}

Li, Y. F. ^{[1
]}

Gao, L. ^{[1
]}

Wang, Zhe ^{[2
]}

Liu, L. ^{[1
,2
]}

Zhu, X. L. ^{[2
]}

机构：

[1] NE Agr Univ, Coll Vet Med, Harbin 150030, Peoples R China

[2] Jilin Univ, Coll Anim Sci & Vet Med, Changchun 130062, Peoples R China

来源：

PAKISTAN VETERINARY JOURNAL | 2012年 / 32卷 / 03期

基金：

美国国家科学基金会;

关键词：

Calf hepatocyte; Insulin receptor; Gene expression; Glucagon; DAIRY-COWS; GLUCONEOGENIC ENZYMES; CALVES; LIVER; SUPPLEMENTATION; METABOLITES; INJECTIONS; GLUCOSE; GROWTH; ALPHA;

D O I：

暂无

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

This study investigated whether increased glucagon levels, caused by the short-term administration of glucagon, lead to an increase in gene expression of the insulin receptor (InsR) in calf hepatocytes cultured in vitro. After 72 hrs of culturing, glucagon was added to calf hepatocytes at a five different concentrations of 0, 1, 10, 100 and 1000 nM. InsR mRNA expression was determined by internally controlled reverse transcriptase polymerase chain reaction. No changes in InsR mRNA expression (InsR/beta-actin gray scale) were detected in hepatocytes treated with glucagon compared with the control group and there were no significant differences between the different concentrations. In conclusion, short-term administration of glucagon did not directly influence the gene expression of InsR in primary cultured calf hepatocytes. (C) 2012 PVJ. All rights reserved

引用

页码：465 / 467

页数：3

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