共 43 条
The human NANOS3 gene contributes to lung tumour invasion by inducing epithelial-mesenchymal transition
被引:16
作者:
Grelet, Simon
[1
]
Andries, Vanessa
[2
,3
]
Polette, Myriam
[1
,4
]
Gilles, Christine
[5
]
Staes, Katrien
[2
,3
]
Martin, Anne-Pascaline
[1
]
Kileztky, Claire
[1
]
Terryn, Christine
[6
]
Dalstein, Veronique
[1
,4
]
Cheng, Chun-Wen
Shen, Chen-Yang
[7
]
Birembaut, Philippe
[1
,4
]
Van Roy, Frans
[2
,3
]
Nawrocki-Raby, Beatrice
[1
]
机构:
[1] Univ Reims, INSERM, UMR S 903, SFR CAP Sante, Champagne Ardenne, France
[2] Univ Ghent, Dept Biomed Mol Biol, B-9000 Ghent, Belgium
[3] Inflammat Res Ctr, Mol Cell Biol Unit, Ghent, Belgium
[4] CHU Reims, Histol Lab, Reims, France
[5] Univ Liege, GIGA Canc, Lab Dev & Tumour Biol, B-4000 Liege, Belgium
[6] Univ Reims, Plateforme Imagerie Cellulaire & Tissulaire, Champagne Ardenne, France
[7] Acad Sinica, Inst Biomed Sci, Taipei 115, Taiwan
关键词:
NANOS3;
lung cancer;
tumour invasion;
epithelial-mesenchymal transition;
post-transcriptional regulation;
SUBCELLULAR-LOCALIZATION;
TRANSLATIONAL REGULATION;
EUKARYOTIC PROTEINS;
DROSOPHILA;
EXPRESSION;
HUNCHBACK;
PUMILIO;
CANCER;
RNA;
RECRUITMENT;
D O I:
10.1002/path.4549
中图分类号:
R73 [肿瘤学];
学科分类号:
100214 ;
摘要:
We have explored the role of the human NANOS3 gene in lung tumour progression. We show that NANOS3 is over-expressed by invasive lung cancer cells and is a prognostic marker for non-small cell lung carcinomas (NSCLCs). NANOS3 gene expression is restricted in testis and brain and is regulated by epigenetic events. It is up-regulated in cultured cells undergoing epithelial-mesenchymal transition (EMT). NANOS3 over-expression in human NSCLC cell lines enhances their invasiveness by up-regulating EMT, whereas its silencing induces mesenchymal-epithelial transition. NANOS3 represses E-cadherin at the transcriptional level and up-regulates vimentin post-transcriptionally. Also, we show that NANOS3 binds mRNAs encoding vimentin and regulates the length of their poly(A) tail. Finally, NANOS3 can also protect vimentin mRNA from microRNA-mediated repression. We thus demonstrate a role for NANOS3 in the acquisition of invasiveness by human lung tumour cells and propose a new mechanism of post-transcriptional regulation of EMT. Copyright (c) 2015 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
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页码:25 / 37
页数:13
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