Doxycycline dependent expression of Epstein-Barr virus latent membrane protein 1 with Tet regulating system in nasopharygeal carcinoma cell line

被引：0

作者：

Liao, W ^{[1
]}

Yi, H ^{[1
]}

Li, XY ^{[1
]}

Tang, M ^{[1
]}

Gu, HH ^{[1
]}

Cao, Y ^{[1
]}

机构：

[1] Hunan Med Univ, Canc Res Inst, Changsha 410078, Peoples R China

来源：

ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 1999年 / 31卷 / 03期

关键词：

Epstein-Barr virus; latent membrane protein 1; regulated expression; nasopharyngeal carcinoma;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) plays a vital role in cell transformation and the nasopharygeal carcinoma (NPC) is highly related to EBV infection. To elucidate whether LMP1 exerts oncogenic effect in NPC and its possible mechanism, a dual-stable LMP1 integrated NPC cell line with Tet-on regulating system were established. Results revealed that expression of LMP-1 could be regulated by Tet system when NPC cell line HNE2 served as receipt cell, and doxycycline could increase this expression by about 10 fold. Therefore, it is possible that different level expression of LMP-1 results in its different biological functions and various efforts on nasopharyngeal carcinogenesis.

引用

页码：309 / 312

页数：4