Wall teichoic acid is an essential component of Staphylococcus aureus for the induction of human dendritic cell maturation

被引：9

作者：

Hong, Sung Jun ^{[1
,2
,6
]}

Kim, Sun Kyung ^{[1
,2
]}

Ko, Eun Byeol ^{[1
,2
]}

Yun, Cheol-Heui ^{[3
,4
,5
]}

Han, Seung Hyun ^{[1
,2
]}

机构：

[1] Seoul Natl Univ, DRI, Dept Oral Microbiol & Immunol, Sch Dent, Bldg 86,1 Gwanak Ro, Seoul 08826, South Korea

[2] Seoul Natl Univ, Sch Dent, Plus Program BK21, Bldg 86,1 Gwanak Ro, Seoul 08826, South Korea

[3] Seoul Natl Univ, Dept Agr Biotechnol, Seoul 08826, South Korea

[4] Seoul Natl Univ, Res Inst Agr & Life Sci, Seoul 08826, South Korea

[5] Seoul Natl Univ, Inst Green Bio Sci Technol, Pyeongchang 25354, South Korea

[6] Mogam Inst Biomed Res, 93 Ihyeon Ro 30 Beon Gil, Yongin 16924, Gyeonggi Do, South Korea

来源：

MOLECULAR IMMUNOLOGY | 2017年 / 81卷

基金：

新加坡国家研究基金会;

关键词：

Staphylococcus aureus; Wall teichoic acid; Mutant; Dendritic cell; Maturation; T cell activation; MANNOSE-BINDING LECTIN; GRAM-POSITIVE BACTERIA; TUMOR-NECROSIS-FACTOR; NOSOCOMIAL INFECTIONS; RESPONSES; SURFACE; POLYSACCHARIDE; COLONIZATION; PHAGOCYTOSIS; PATHOGENESIS;

D O I：

10.1016/j.molimm.2016.12.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Staphylococcus aureus is a Gram-positive pathogen that can cause chronic skin inflammation, pneumonia, and septic shock. The immunomodulatory functions of wall teichoic acid (WTA), a glycopolymer abundantly expressed on the Gram-positive bacterial cell wall, are poorly understood. Here, we investigated the role of WTA in the phenotypic and functional activation of human monocyte-derived dendritic cells (DCs) treated with ethanol-killed S. aureus. WTA-deficient S. aureus mutant (Delta tagO) exhibited attenuated binding and internalization to DCs compared to the wild-type. Delta tagO induced lower expression of maturation markers on and cytokines in DCs than the wild-type S. aureus. Furthermore, autologous human peripheral blood mononuclear cells cocultured with Delta tagO-treated DCs exhibited a marked reduction in T cell proliferative activity, the expression of activation markers, and the production of cytokines compared to the wild-type S. aureus-stimulated DCs. Collectively, these results suggest that WTA is an important cell wall component of S. aureus for the induction of DC maturation and activation. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：135 / 142

页数：8

共 43 条

[31] PRODUCTION OF INTERLEUKIN-1 BUT NOT TUMOR NECROSIS FACTOR BY HUMAN-MONOCYTES STIMULATED WITH PNEUMOCOCCAL CELL-SURFACE COMPONENTS [J].