The paper investigates whether gender and/or personality traits influence innovativeness, based on questionnaire (N= 264, conducted in December 2016-January 2017) among university students from the Czech Republic. The Big Five Inventory (BFI); a self-report inventory designed to measure five personality traits (extraversion, agreeableness, conscientiousness, neuroticism, openness to experience) was used. A general linear model was used to analyze impact of gender and BFI on innovativeness. It is a replication. Results confirm that all but agreaableness had significant impact on self-perceived innovativeness: according to a linear regression model, extraversion, conscientiousness, neuroticism, openness to experience, and gender influence self-perceived innovativeness. Impact of extraversion, conscientiousness, and openness to experience is positive. Impact of neuroticism is negative. And men rate their innovativeness higher than women, controlling for personality traits.