A magnetic chitosan hydrogel for sustained and prolonged delivery of Bacillus Calmette-Guerin in the treatment of bladder cancer

被引：119

作者：

Zhang, Dong ^{[1
]}

Sun, Peng ^{[1
]}

Li, Peng ^{[1
]}

Xue, Aibing ^{[1
]}

Zhang, Xiaokai ^{[2
]}

Zhang, Haiyang ^{[1
,3
]}

Jin, Xunbo ^{[1
]}

机构：

[1] Shandong Univ, Shandong Prov Hosp, Minimally Invas Urol Ctr, Jinan 250021, Peoples R China

[2] Shandong Normal Univ, Coll Phys & Elect, Electron Microscope Lab, Jinan 250014, Peoples R China

[3] Univ Calif San Francisco, Sch Med, Dept Urol, Knuppe Mol Urol Lab, San Francisco, CA 94143 USA

来源：

BIOMATERIALS | 2013年 / 34卷 / 38期

基金：

中国国家自然科学基金; 国家高技术研究发展计划(863计划);

关键词：

Bacillus Calmette-Guerin; Intravesical instillation; Chitosan; Magnetic nanoparticle; Thermosensitive hydrogel; Intravesical drug delivery; DRUG-DELIVERY; INTRAVESICAL IMMUNOTHERAPY; THERMOSENSITIVE HYDROGEL; UROTHELIAL CARCINOMA; BCG IMMUNOTHERAPY; CHEMOTHERAPY; SYSTEM; NANOPARTICLES; INSTILLATION; DOXORUBICIN;

D O I：

10.1016/j.biomaterials.2013.09.027

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The aim of this study was to develop a magnetic thermosensitive hydrogel as intravesical Bacillus Calmette-Guerin (BCG) delivery system, which was formulated with chitosan (CS), beta-glycerophosphate (GP) and Fe3O4 magnetic nanoparticle (Fe3O4-MNP). The gelation time and magnetic response of the gel system were investigated. The morphology of the gel was displayed by scanning electron microscope. Frozen section examination was creatively employed for exhibiting the structure of the gel and determining its intravesical residence time. The antitumor effect and local immune activity of BCG loaded magnetic gel were evaluated. The flowing solution of CS/GP under room temperature could gelate rapidly at body temperature both in vitro and in vivo. The magnetic injectable hydrogels significantly prolonged intravesical BCG residence time under an applied magnetic field. In comparison to traditional BCG therapy for superficial bladder tumor, BCG delivered by the gel system induced a stronger Th1 immune response and revealed higher antitumor efficacy. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：10258 / 10266

页数：9

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