Intergeneric conjugal transfer of plasmid DNA from Escherichia coli to Kitasatospora setae, a bafilomycin B1 producer

被引：58

作者：

Choi, SU

Lee, CK

Hwang, YI

Kinoshita, H

Nihira, T

机构：

[1] Osaka Univ, Int Ctr Biotechnol, Suita, Osaka 5650871, Japan

[2] Kyungnam Univ, PrimBioRandDs, Inst Genome Reconstruct, Masan 631701, South Korea

[3] Kyungnam Univ, Dept Food Sci & Biotechnol, Div Life Sci, Masan 631701, South Korea

来源：

ARCHIVES OF MICROBIOLOGY | 2004年 / 181卷 / 04期

关键词：

conjugal transfer; integration site; Kitasatospora setae; bafilomycin B-1;

D O I：

10.1007/s00203-004-0654-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An effective transformation procedure for Kitasatospora setae was established based on transconjugation from Escherichia coli ET12567 (pUZ8002) using a phiC31-derived integration vector, pSET152, containing oriT and attP fragments. While no transconjugation was observed under the standard transconjugation conditions for Streptomyces species, sufficient transconjugation (>1x10(-6)) was achieved on ISP4 medium containing 30 mM MgCl2 using a 25- to 125-fold excess of E. coli donor cells. In addition, the sequence and location of the chromosomal integration site attB of K. setae was identified for the first time in genera of non-Streptomyces actinomycetes. K. setae contains a single phiC31 attB site. Similar to the case of Streptomyces species, the attB site of K. setae is present within an ORF encoding a pirin-homolog, but the K. setae-attB sequence deviates slightly from the consensus sequence of Streptomyces attB sequences.

引用

页码：294 / 298

页数：5

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