Experimental Study on Low-Detection Limit for Immunomagnetic Reduction Assays by Manipulating Reagent Entities

被引：11

作者：

Yang, S. Y. ^{[1
,2
]}

Yang, C. C. ^{[2
]}

Horng, H. E. ^{[2
]}

Shih, B. Y. ^{[2
]}

Chieh, J. J. ^{[2
]}

Hong, C. Y. ^{[3
]}

Yang, H. C. ^{[4
,5
]}

机构：

[1] MagQu Co Ltd, New Taipei City 231, Taiwan

[2] Natl Taiwan Normal Univ, Inst Electroopt Sci & Technol, Taipei 116, Taiwan

[3] Natl Chung Hsing Univ, Grad Inst Biomed Engn, Taichung 402, Taiwan

[4] Natl Taiwan Univ, Dept Phys, Taipei 106, Taiwan

[5] Kun Shan Univ, Dept Electroopt Engn, Tainan 710, Taiwan

来源：

IEEE TRANSACTIONS ON NANOBIOSCIENCE | 2013年 / 12卷 / 02期

关键词：

Immunomagnetic reduction; magnetic nanoparticles; reagent; PARTICLES;

D O I：

10.1109/TNB.2013.2240009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The low limit of detection (LLD) plays an important role in biomolecular assays, especially for early-stage assays. Biomolecular detections usually involve the use of two main elements: a reagent and an analyzer, which both greatly contribute to the LLD. In this work, the relationships among the LLD and reagent-related factors are investigated. The to-be-detected biomolecule is c-reactive protein (CRP) as an example. The assay method is immunomagnetic reduction (IMR). The components of reagent Fe3O4 are magnetic nanoparticles bio-functionalized with antibodies against CRP, dispersed in pH-7.4 phosphate buffered saline solution. Several key factors of the reagent, such as particle concentration, volume ratio of reagent to sample, and particle size, are manipulated to optimize the LLD of detecting CRP.

引用

页码：65 / 68

页数：4

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