Label-free detection of single-stranded DNA binding protein based on a cantilever array

被引：10

作者：

Hou, Hui ^{[1
,2
]}

Bai, Xiaojing ^{[2
,3
]}

Xing, Chunyan ^{[2
,3
]}

Lu, Baoping ^{[2
,3
]}

Hao, Jinhui ^{[2
,3
]}

Ke, Xi ^{[2
,3
]}

Gu, Ningyu ^{[1
]}

Zhang, Bailin ^{[2
]}

Tang, Jilin ^{[2
]}

机构：

[1] Nanchang Univ, Dept Chem, Nanchang 330031, Peoples R China

[2] Changchun Inst Appl Chem, Chinese Acad Sci, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China

[3] Univ Chinese Acad Sci, Beijing 100039, Peoples R China

来源：

TALANTA | 2013年 / 109卷

基金：

中国国家自然科学基金;

关键词：

Cantilever array sensor; Self-assembly; Single-stranded DNA (ssDNA); Single-stranded DNA-binding protein (SSBP); Specificity; ESCHERICHIA-COLI SSB; MODES; ENTHALPY; SENSORS; DOMAIN;

D O I：

10.1016/j.talanta.2013.02.001

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report a simple and sensitive method for label-free detection of single-stranded DNA-binding protein (SSBP) based on an array of microfabricated cantilevers. The single-stranded DNA (ssDNA) was immobilized on the surface of the sensing cantilevers to detect SSBP, while the reference cantilevers were modified with 6-mercapto-1-hexanol to detect any unwanted cantilever deflection. The differential deflection signals that reveal specific SSBP-ssDNA binding have been found to depend on the SSBP concentration. Using the cantilever array sensor we can detect SSBP in the concentration range from 0.01 to 7 mu g mL(-1). Other proteins, such as thrombin or bovine serum albumin induced no significant deflection of the cantilevers. Our results show the potential for the application of cantilever array sensor system as a powerful tool to detect proteins with high sensitivity and specificity. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：173 / 176

页数：4

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