A 3-O-methylated mannogalactan from Pleurotus pulmonarius: Structure and antinociceptive effect

被引：72

作者：

Smiderle, F. R. ^{[1
]}

Olsen, L. M. ^{[1
]}

Carbonero, E. R. ^{[1
]}

Marcon, R. ^{[2
]}

Baggio, C. H. ^{[3
]}

Freitas, C. S. ^{[3
]}

Santos, A. R. S. ^{[2
]}

Torri, G. ^{[4
]}

Gorin, P. A. J. ^{[1
]}

Iacomini, M. ^{[1
,5
]}

机构：

[1] Univ Fed Parana, Dept Bioquim & Biol Mol, BR-81531980 Curitiba, Parana, Brazil

[2] Univ Fed Santa Catarina, Ctr Ciencias Biol, Dept Ciencias Fisiol, BR-88040900 Florianopolis, SC, Brazil

[3] Univ Fed Parana, Dept Farmacol, BR-81531980 Curitiba, Parana, Brazil

[4] Ist Ric Chim & Biochim G Ronzoni, I-20133 Milan, Italy

[5] Fac Doutor Leocadio Jose Correia, Dept Pedagogia, BR-82640070 Curitiba, Parana, Brazil

来源：

PHYTOCHEMISTRY | 2008年 / 69卷 / 15期

关键词：

Pleurotus pulmonarius; Basidiomycete; Polysaccharide; Mannogalactan; Antinociceptive effect;

D O I：

10.1016/j.phytochem.2008.08.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A polysaccharide (M-w 2.39 x 10(4) g/mol) was extracted with cold water from the basidiomycete Pleurotus pulmonarius, and its antinociceptive and anti-inflammatory properties were evaluated. It was a mannogalactan (MG), whose structure was characterized using mono- and two-dimensional NMR spectroscopy, methylation analysis, and a controlled Smith degradation. It had a main chain of (1 -> 6)-linked alpha-D-galactopyranosyl and 3-O-methyl-alpha-D-galactopyranosyl units, both of which are partially substituted at O-2 by beta-D-mannopyranosyl non-reducing ends. The MG was tested for its effects on the acetic acid-induced writhing reaction in mice, a typical model for inflammatory pain, causing a marked and dose-dependent inhibition of the nociceptive response, with ID50 of 16.2 (14.7-17.7) mg/kg and inhibition of 93 +/- 3% at a dose of 30 mg/kg. An inflammatory response was not inhibited. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：2731 / 2736

页数：6

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