A new Kaliotoxin selective towards Kv1.3 and Kv1.2 but not Kv1.1 channels expressed in oocytes

被引：23

作者：

Abbas, Najwa ^{[1
,2
]}

Belghazi, Maya ^{[3
]}

Abdel-Mottaleb, Yousra ^{[4
]}

Tytgat, Jan ^{[4
]}

Bougis, Pierre E. ^{[1
,2
]}

Martin-Eauclaire, Marie-France ^{[1
,2
]}

机构：

[1] Univ Aix Marseille 2, CNRS, UMR 6231, CRN2M, F-13916 Marseille, France

[2] Univ Paul Cezanne, CNRS, UMR 6231, CRN2M, F-13916 Marseille, France

[3] Univ Aix Marseille 2, CAPM, Inst Jean Roche, F-13916 Marseille, France

[4] Univ Louvain, Toxicol Lab, B-3000 Louvain, Belgium

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 376卷 / 03期

关键词：

Scorpion toxins; Kaliotoxin; Voltage-gated potassium channels;

D O I：

10.1016/j.bbrc.2008.09.033

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this paper were described the purification, the sequencing, and the immunological biological characterization of a new Kaliotoxin analog, Aam-KTX, from the venom of the scorpion Androctonus amoreuxi The toxin effects on three cloned Kv channels (Kv1.1, Kv1.2, and Kv1.3) were investigated in Xenopus oocytes using electrophysiology experiments. The Aam-KTX preference for Kv1.3 channel versus Kv1.2 was expected (EC50 values, 1.1 +/- 0.02 and 10.4 +/- 1.5 nM, respectively) but its total inefficacity on Kv1.1 was very surprising. 3D molecular modeling of Aam-KTX brought putative answers to this difference in selectivity. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：525 / 530

页数：6

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