Quencher-free linear probe with multiple fluorophores on an acyclic scaffold

被引：30

作者：

Asanuma, Hiroyuki ^{[1
]}

Akahane, Mariko ^{[1
]}

Kondo, Nobuyo ^{[1
]}

Osawa, Takuya ^{[1
]}

Kato, Tomohiro ^{[1
]}

Kashida, Hiromu ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Engn, Chikusa Ku, Nagoya, Aichi 4648603, Japan

来源：

CHEMICAL SCIENCE | 2012年 / 3卷 / 11期

基金：

日本科学技术振兴机构;

关键词：

MOLECULAR BEACONS; LEUKEMIA-CELLS; DNA; HYBRIDIZATION; STEM; DESIGN; DUPLEX;

D O I：

10.1039/c2sc20732j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have developed a new quencher-free stemless linear probe involving multiple perylenes incorporated through D-threoninol; each perylene is separated by intervening natural nucleotides. Without a substrate, the flexible linear probe does not emit fluorescence due to the self-quenching of the weakly interacting fluorophores. Upon hybridization with the target, intercalation of each dye between the base pairs results in emission of strong fluorescence. The maximum signal-background ratio attained was 180, and the response rate was significantly faster than that of a classic hairpin-forming molecular beacon.

引用

页码：3165 / 3169

页数：5

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