Microbial Fucoidan Degradation by Luteolibacter algae H18 with Deacetylation

被引：23

作者：

Ohshiro, Takashi ^{[1
]}

Harada, Naomi ^{[1
]}

Kobayashi, Yasuaki ^{[1
]}

Miki, Yasunari ^{[2
]}

Kawamoto, Hitoshi ^{[2
]}

机构：

[1] Tottori Univ, Dept Biotechnol, Tottori 6808552, Japan

[2] Marine Prod Kimuraya Co Ltd, Tottori 6840072, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2012年 / 76卷 / 03期

关键词：

fucoidan; deacetylase; Luteolibacter; Cladosiphon okamuranus (Okinawamozuku); MARINE BACTERIAL STRAIN; SULFATED FUCANS; CELL-LINES; DIGESTION;

D O I：

10.1271/bbb.110911

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A bacterial strain that assimilates fucoidan from Cladosiphon okamuranus as sole carbon source was isolated as Luteolibacter algae H-18. It was found that it degraded fucoidan by intracellular enzymes, and that the degradation reactions were catalyzed by multiple enzymes. One enzyme, designated fraction B, was established to exhibit the deacetylation reaction of fucoidan. Other enzyme(s), designated fraction A, catalyzed the reaction(s) lowering the molecular weight of fucoidan.

引用

页码：620 / 623

页数：4

共 19 条

[1] Cloning and biochemical characterization of the fucanase FcnA: Definition of a novel glycoside hydrolase family specific for sulfated fucans [J].

Colin, Sebastien ;

Deniaud, Estelle ;

Jam, Murielle ;

Descamps, Valerie ;

Chevolot, Yann ;

Kervarec, Nelly ;

Yvin, Jean-Claude ;

Barbeyron, Tristan ;

Michel, Gurvan ;

Kloareg, Bernard .

GLYCOBIOLOGY, 2006, 16 (11) :1021-1032

[2] Isolation and culture of a marine bacterium degrading the sulfated fucans from marine brown algae [J].

Descamps, V ;

Colin, S ;

Lahaye, M ;

Jam, M ;

Richard, C ;

Potin, P ;

Barbeyron, T ;

Yvin, JC ;

Kloareg, B .

MARINE BIOTECHNOLOGY, 2006, 8 (01) :27-39

[3] DETERMINATION OF REDUCING SUGAR WITH IMPROVED PRECISION [J].

DYGERT, S ;

LI, LH ;

FLORIDA, D ;

THOMA, JA .

ANALYTICAL BIOCHEMISTRY, 1965, 13 (03) :367-&

[4]

Fukahori S, 2008, MOL MED REP, V1, P537

[5] Structure and anti-dengue virus activity of sulfated polysaccharide from a marine alga [J].

Hidari, Kazuya I. P. J. ;

Takahashi, Naonori ;

Arihara, Masataka ;

Nagaoka, Masato ;

Morita, Koichi ;

Suzuki, Takashi .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2008, 376 (01) :91-95

[6]

HIRAISHI A, 1995, B JPN SOC MICROB ECO, V10, P31

[7] Effects of fucoidan from Mozuku on human stomach cell lines [J].

Kawamoto, Hitoshi ;

Miki, Yasunari ;

Kimura, Takayuki ;

Tanaka, Katsunori ;

Nakagawa, Tsuyoshi ;

Kawamukai, Makoto ;

Matsuda, Hideyuki .

FOOD SCIENCE AND TECHNOLOGY RESEARCH, 2006, 12 (03) :218-222

[8] A novel bacterial ATP-binding cassette transporter system that allows uptake of macromolecules [J].

Momma, K ;

Okamoto, M ;

Mishima, Y ;

Mori, S ;

Hashimoto, W ;

Murata, K .

JOURNAL OF BACTERIOLOGY, 2000, 182 (14) :3998-4004

[9] Use of sulfated fucans as anticoagulant and antithrombotic agents: Future perspectives [J].

Mourao, PAS .

CURRENT PHARMACEUTICAL DESIGN, 2004, 10 (09) :967-981

[10] Structural study of fucoidan from Cladosiphon okamuranus TOKIDA [J].

Nagaoka, M ;

Shibata, H ;

Kimura-Takagi, I ;

Hashimoto, S ;

Kimura, K ;

Makino, T ;

Aiyama, R ;

Ueyama, S ;

Yokokura, T .

GLYCOCONJUGATE JOURNAL, 1999, 16 (01) :19-26

← 1 2 →