Effect of tamoxifen on extracellular signal-regulated kinases in the urethra of castrated female rats

被引：1
|
作者：
dos Santos, Alesse R. [1 ]
Lopes-Costa, Pedro V. [1 ]
Gontijo, Jose A. [2 ]
da Silva, Benedito B. [1 ]
机构：
[1] Univ Fed Piaui, Dept Gynecol, Teresina, Piaui, Brazil
[2] Univ Estadual Campinas, Campinas, SP, Brazil
来源：
EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY | 2012年 / 164卷 / 01期
关键词：
ERKs; Protein kinases; Rats; Tamoxifen; Urethra; ACTIVATED PROTEIN-KINASE; ESTROGEN-RECEPTOR; BREAST-CANCER; CELLS; PROLIFERATION; PHOSPHORYLATION; EXPRESSION; PATHWAYS; CASCADE; ERK;
D O I：
10.1016/j.ejogrb.2012.05.036
中图分类号：
R71 [妇产科学];
学科分类号：
100211 ;
摘要：
Objective: The aim was to evaluate the effects of tamoxifen in activating extracellular signal-regulated kinases (ERKs) 1 and 2 in the urethras of castrated female rats. Study design: Twelve castrated adult female rats were divided into a control group (n = 6) in which the animals received vehicle, and the experimental group (n = 6) in which the rats received tamoxifen 250 mu g/day by gavage for 28 days. Then, the animals were sacrificed and their urethras removed. Proteins were extracted, quantified and processed by Western blot analysis with specific phospho-ERK1 and 2 antibodies. Data were analyzed using Student's t-test (p < 0.05). Results: A significant increase occurred in phospho-ERK1 levels in the experimental group compared to the control group (p < 0.01), while no difference was found in phospho-ERK2 levels between the groups (p = 0.313). Conclusion: The present results indicate that, at the doses and during the time of treatment used, tamoxifen significantly increased phospho-ERK1 levels in the urethras of castrated female rats. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：102 / 104
页数：3
相关论文
共 50 条
  • [21] Activation of extracellular signal-regulated kinases potentiates hemin toxicity in astrocyte cultures
    Regan, RF
    Wang, YZ
    Ma, X
    Chong, A
    Guo, YP
    JOURNAL OF NEUROCHEMISTRY, 2001, 79 (03) : 545 - 555
  • [22] Extracellular signal-regulated kinases regulate dendritic growth in rat sympathetic neurons
    Kim, IJ
    Drahushuk, KM
    Kim, WY
    Gonsiorek, EA
    Lein, P
    Andres, DA
    Higgins, D
    JOURNAL OF NEUROSCIENCE, 2004, 24 (13): : 3304 - 3312
  • [23] Regulation of the extracellular signal-regulated kinases following acute and chronic opioid treatment
    Bilecki, W
    Zapart, G
    Ligeza, A
    Wawrzczak-Bargiela, A
    Urbanski, MJ
    Przewlocki, R
    CELLULAR AND MOLECULAR LIFE SCIENCES, 2005, 62 (19-20) : 2369 - 2375
  • [24] Bradykinin activation of extracellular signal-regulated kinases in human trabecular meshwork cells
    Webb, Jerry G.
    Yang, Xiaofeng
    Crosson, Craig E.
    EXPERIMENTAL EYE RESEARCH, 2011, 92 (06) : 495 - 501
  • [25] Salicylate inhibition of extracellular signal-regulated kinases and inducible nitric oxide synthase
    Wang, ZY
    Brecher, P
    HYPERTENSION, 1999, 34 (06) : 1259 - 1264
  • [26] Isoprotrenol activates extracellular signal-regulated protein kinases in cardiomyocytes through calcineurin
    Shizukuda, Y
    Buttrick, PM
    CIRCULATION, 2002, 105 (02) : E9 - E9
  • [27] Extracellular signal-regulated kinases associate with and phosphorylate DHPS to promote cell proliferation
    Wang, Chao
    Chen, Zhen
    Nie, Litong
    Tang, Mengfan
    Feng, Xu
    Su, Dan
    Zhang, Huimin
    Xiong, Yun
    Park, Jeong-Min
    Chen, Junjie
    ONCOGENESIS, 2020, 9 (09)
  • [28] Isoproterenol activates extracellular signal-regulated protein kinases in cardiomyocytes through calcineurin
    Zou, YZ
    Yao, A
    Zhu, WD
    Kudoh, S
    Hiroi, Y
    Shimoyama, M
    Uozumi, H
    Kohmoto, O
    Takahashi, T
    Shibasaki, F
    Nagai, R
    Yazaki, Y
    Komuro, I
    CIRCULATION, 2001, 104 (01) : 102 - 108
  • [29] Native and modified LDL activate extracellular signal-regulated kinases in mesangial cells
    Jenkins, AJ
    Velarde, V
    Klein, RL
    Joyce, KC
    Phillips, KD
    Mayfield, RK
    Lyons, TJ
    Jaffa, AA
    DIABETES, 2000, 49 (12) : 2160 - 2169
  • [30] How does hydrogen peroxide activate extracellular signal-regulated protein kinases?
    Pang, WW
    Lui, J
    Arthur, P
    FASEB JOURNAL, 2004, 18 (08): : C35 - C35
12345