Electrochemical aptasensor using boron-carbon nanorods decorated by nickel nanoparticles for detection of E. coli O157:H7

被引：28

作者：

Kaur, Harmanjit ^{[1
]}

Shorie, Munish ^{[1
]}

Sabherwal, Priyanka ^{[1
,2
]}

机构：

[1] Inst Nano Sci & Technol, Mohali 160062, India

[2] Univ Mumbai, Natl Ctr Nanosci & Nanotechnol, Mumbai 400098, Maharashtra, India

来源：

MICROCHIMICA ACTA | 2020年 / 187卷 / 08期

关键词：

Pathogen detection; E; coli; O157:H7; Aptasensor; Nanosensor; Boron-carbon nanostructures; LABEL-FREE DETECTION; CITROBACTER-FREUNDII; GRAPHENE; POLYSACCHARIDE; NANOSHEETS; GRAPHITE; COATINGS; STRAINS;

D O I：

10.1007/s00604-020-04444-y

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The development of a label-free impedimetric aptasensor is reported for rapid and sensitive detection of Escherichia coli O157:H7 employing boron-carbon nanorods decorated by nickel nanoparticles (BC-Ni) nanostructured platform. These highly electroactive BC-Ni nanorods were synthesized to increase the sensitivity of the sensor surface and subsequently functionalized with a specific anti-E. coli O157:H7 aptamer (K-d = 69 nM) as bio-recognition moiety. This fully characterized high-affinity DNA aptamer against the bacteria was selected using a facile microtiter plate-based cell-SELEX methodology. The fabricated electrochemical aptasensor is demonstrated to detect E. coli O157:H7 selectively with a detection limit of 10 cfu and a dynamic detection range of 10(0) to 10(5) cfu in water, juice, and fecal samples.

引用

页数：10

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