Regeneration of transgenic Picea glauca, P-mariana, and P-abies after cocultivation of embryogenic tissue with Agrobacterium tumefaciens

被引：0
作者：
Klimaszewska, K
Lachance, D
Pelletier, G
Lelu, MA
Séguin, A
机构：
[1] Nat Resources Canada, Canadian Forestry Serv, Laurentian Forestry Ctr, Ste Foy, PQ G1V 4C7, Canada
[2] INRA, CRO, Unite Ameliorat Genet & Physiol Forestieres, F-15166 Olivet, France
来源：
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT | 2001年 / 37卷 / 06期
关键词：
black spruce; cocultivation; Norway spruce; white spruce; somatic embryogenesis; transgenic plants;
D O I：
暂无
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Transgenic plants of three Picea species were produced after coculture of embryogenic tissue with the disarmed strain of Agrobacterium tumefaciens C58/pMP90/pBIV10 and selection on medium containing kanamycin. In addition to the nptII selectable gene (conferring resistance to kanamycin), the vector carried the uidA (beta -glucuronidase) marker gene, Transformation frequencies were dependent on the species, genotype, and post-cocultivation procedure. Of the three species tested, P. mariana was transformed at the highest frequency, followed by P. glauca and P. abies. The transgenic state of the embryogenic tissue was initially confirmed by histochemical beta -glucuronidase (GLIS)assay followed ky Southern hybridization. One to over five copies of T-DNA were detected in various transgenic lines analyzed. Transgenic plants were regenerated for all species using modified protocols for maturation and germination of somatic embryos.
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收藏
页码：748 / 755
页数：8
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