R-loops cause replication impairment and genome instability during meiosis

被引：65

作者：

Castellano-Pozo, Maikel ^{[1
]}

Garcia-Muse, Tatiana ^{[1
]}

Aguilera, Andres ^{[1
]}

机构：

[1] Univ Seville, CSIC, Ctr Andaluz Biol Mol & Med Regenerat CABIMER, Seville 41092, Spain

来源：

EMBO REPORTS | 2012年 / 13卷 / 10期

关键词：

R-loops; Saccharomyces cerevisiae; Caenorhabditis elegans; meiotic replication; genome instability; DOUBLE-STRAND BREAKS; CAENORHABDITIS-ELEGANS; THO COMPLEX; C-ELEGANS; MEIOTIC RECOMBINATION; CHROMOSOME SYNAPSIS; CELL-CYCLE; TRANSCRIPTION; YEAST; ACTIVATION;

D O I：

10.1038/embor.2012.119

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

R-loops are harmful structures with a negative impact on transcription and recombination during mitosis, but no information exists for meiosis. We used Saccharomyces cerevisiae and Caenorhabditis elegans THO mutants as a tool to determine the consequences of R-loops in meiosis. We found that both S. cerevisiae and C. elegans THO mutants show defective meiosis and an impairment of premeiotic replication as well as DNA-damage accumulation. Importantly, RNase H partially suppressed the replication impairment and the DNA-damage accumulation. We conclude that R-loops can form during meiosis causing replication impairment with deleterious results.

引用

页码：923 / 929

页数：7

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