A bioactive nano-calcium phosphate paste for in-situ transfection of BMP-7 and VEGF-A in a rabbit critical-size bone defect: results of an in vivo study

被引：37

作者：

Schlickewei, Carsten ^{[1
]}

Klatte, Till O. ^{[1
]}

Wildermuth, Yasmin ^{[1
]}

Laaff, Georg ^{[1
]}

Rueger, Johannes M. ^{[1
]}

Ruesing, Johannes ^{[2
,3
]}

Chernousova, Svitlana ^{[2
,3
]}

Lehmann, Wolfgang ^{[4
]}

Epple, Matthias ^{[2
,3
]}

机构：

[1] Univ Med Ctr Hamburg Eppendorf, Dept Trauma Hand & Reconstruct Surg, Martinistr 52, D-20246 Hamburg, Germany

[2] Univ Duisburg Essen, Inorgan Chem, Univ Str 5-7, D-45117 Essen, Germany

[3] Univ Duisburg Essen, Ctr Nanointegrat Duisburg Essen CeNIDE, Univ Str 5-7, D-45117 Essen, Germany

[4] Univ Hosp Gottingen, Dept Trauma Orthopaed & Plast Surg, Robert Koch Str 40, D-37075 Gottingen, Germany

来源：

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE | 2019年 / 30卷 / 02期

关键词：

ENDOTHELIAL GROWTH-FACTOR; NONVIRAL GENE-THERAPY; ILIAC CREST; MORPHOGENETIC PROTEINS; NUCLEIC-ACIDS; DELIVERY; NANOPARTICLES; DNA; CELLS; VITRO;

D O I：

10.1007/s10856-019-6217-y

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The aim of this study was to prepare an injectable DNA-loaded nano-calcium phosphate paste that is suitable as bioactive bone substitution material. For this we used the well-known potential of calcium phosphate in bone contact and supplemented it with DNA for the in-situ transfection of BMP-7 and VEGF-A in a critical-size bone defect. 24 New Zealand white rabbits were randomly divided into two groups: One group with BMP-7- and VEGF-A-encoding DNA on calcium phosphate nanoparticles and a control group with calcium phosphate nanoparticles only. The bone defect was created at the proximal medial tibia and filled with the DNA-loaded calcium phosphate paste. As control, a bone defect was filled with the calcium phosphate paste without DNA. The proximal tibia was investigated 2, 4 and 12 weeks after the operation. A histomorphological analysis of the dynamic bone parameters was carried out with the Osteomeasure system. The animals treated with the DNA-loaded calcium phosphate showed a statistically significantly increased bone volume per total volume after 4 weeks in comparison to the control group. Additionally, a statistically significant increase of the trabecular number and the number of osteoblasts per tissue area were observed. These results were confirmed by radiological analysis. The DNA-loaded bone paste led to a significantly faster healing of the critical-size bone defect in the rabbit model after 4 weeks. After 12 weeks, all defects had equally healed in both groups. No difference in the quality of the new bone was found. The injectable DNA-loaded calcium phosphate paste led to a faster and more sustained bone healing and induced an accelerated bone formation after 4 weeks. The material was well integrated into the bone defect and new bone was formed on its surface. The calcium phosphate paste without DNA led to a regular healing of the critical-size bone defect, but the healing was slower than the DNA-loaded paste. Thus, the in-situ transfection with BMP-7 and VEGF-A significantly improved the potential of calcium phosphate as pasty bone substitution material. [GRAPHICS] .

引用

页数：12

共 83 条

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