Direct biocatalytic one-pot-transformation of cyclohexanol with molecular oxygen into ε-caprolactone

被引：67

作者：

Staudt, Svenja ^{[1
]}

Bornscheuer, Uwe T. ^{[2
]}

Menyes, Ulf ^{[3
]}

Hummel, Werner ^{[4
]}

Groeger, Harald ^{[1
,5
]}

机构：

[1] Univ Erlangen Nurnberg, Dept Chem & Pharm, D-91054 Erlangen, Germany

[2] Inst Biochem, Dept Biotechnol & Enzyme Catalysis, D-17487 Greifswald, Germany

[3] Enzym AG, D-17489 Greifswald, Germany

[4] Univ Dusseldorf, Res Ctr Julich, Inst Mol Enzyme Technol, D-52426 Julich, Germany

[5] Univ Bielefeld, Fac Chem, D-33615 Bielefeld, Germany

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2013年 / 53卷 / 04期

关键词：

Alcohol dehydrogenase; Baeyer-Villiger monooxygenase; Cyclohexanol; Cyclohexanone; epsilon-Caprolactone; Oxidation; BAEYER-VILLIGER MONOOXYGENASES;

D O I：

10.1016/j.enzmictec.2013.03.011

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The development of a biocatalytic process concept for epsilon-caprolactone, which directly converts cyclohexanol as an easily available industrial raw material into the desired epsilon-caprolactone in a one-pot fashion while only requiring air as sole reagent, is reported. The desired product epsilon-caprolactone was obtained with 94-97% conversion when operating at a substrate concentration in the range of 20-60 mM. At higher substrate concentrations, however, a significant drop of conversion was found. Subsequent detailed studies on the impact of the starting material, intermediate and product components revealed a significant inhibition and partial deactivation of the BVMO by the product epsilon-caprolactone (in particular at higher concentrations) as well as an inhibition of the BVMO by cyclohexanol and cyclohexanone. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：288 / 292

页数：5

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