Comparison of multiplex polymerase chain reaction and immunoassay to detect Salmonella spp., S. Typhimurium, and S. Enteritidis in Thai chicken meat

被引：10
作者：
Rodpai, Ekkarat [1 ]
Moongkarndi, Prinichanien [1 ]
Tungrugsasut, Walla [2 ]
Phisannoradej, Rangsichon [1 ]
Kanarat, Sasitorn [3 ]
机构：
[1] Mahidol Univ, Fac Pharm, Dept Microbiol, Bangkok 10700, Thailand
[2] Mahidol Univ, Fac Pharm, Dept Food Chem, Bangkok 10700, Thailand
[3] Minist Agr & Cooperat, Dept Livestock Dev, Vet Publ Hlth Lab, Bangkok, Thailand
来源：
SCIENCEASIA | 2013年 / 39卷 / 02期
关键词：
enzyme-linked immunosorbent assay (ELISA); immunomagnetic separation (IMS); food safety; CAMPYLOBACTER-JEJUNI; ESCHERICHIA-COLI; RAPID DETECTION; PCR ASSAY; PATHOGENS; ELISA; IDENTIFICATION;
D O I：
10.2306/scienceasia1513-1874.2013.39.150
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The multiplex PCR (mPCR), enzyme-linked immunosorbent assay (ELISA) and immunomagnetic separation enzyme-linked immunosorbent assay (IMS-ELISA) were developed for the detection of Salmonella spp. and two specific serovars S. Typhimurium and S. Enteritidis. The detection limits of the mPCR without the pre-enriched step was at 10(5) cfu/ml, whereas developed immunoassays demonstrated positive results at 10(5), 10(6), and 10(7) cfu/ml by ELISA and at 10(4), 10(4), and 10(6) cfu/ml by IMS-ELISA for detecting Salmonella spp., S. Typhimurium, and S. Enteritidis, respectively. The mPCR did not produce any nonspecific amplified products when tested against other related species of bacteria. In this study, the developed IMS-ELISA gave more sensitivity but less specificity than the mPCR assay. The methods are useful for the rapid detection of salmonellae in naturally infected chicken meat in Thailand.
引用
收藏
页码：150 / 159
页数：10
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