Evaluation of a rapid method for the quantitative analysis of fatty acids in various matrices

被引：103

作者：

Araujo, Pedro ^{[1
]}

Nguyen, Thu-Thao ^{[1
]}

Froyland, Livar ^{[1
]}

Wang, Jingdong ^{[2
,3
]}

Kang, Jing X. ^{[2
,3
]}

机构：

[1] Natl Inst Nutr & Seafood Res NIFES, N-5817 Bergen, Norway

[2] Massachusetts Gen Hosp, Dept Med, Boston, MA 02114 USA

[3] Harvard Univ, Sch Med, Boston, MA 02114 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2008年 / 1212卷 / 1-2期

关键词：

Methylation; Methylation models; Gas chromatography; High performance thin layer chromatography;

D O I：

10.1016/j.chroma.2008.10.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simplified method for quantitative analysis of fatty acids in various matrices by gas chromatography is proposed as an alternative to the conventional method and the variables of the protocol examined to optimize the processing conditions. The modified method involves direct methylation of fatty acids in homogenized samples with boron trihalide (BF3 or BCl3 in methanol) followed by extraction with hexane. The addition of hexane to the reaction mixture after the methylation process can enhance the efficiency of fatty acid methylation and is critical for those samples that contain high levels of triglycerides. A mechanism underlying this effect is proposed. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：106 / 113

页数：8

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