LOW YIELD BY MOLECULAR DETECTION OF CHLAMYDOPHILA PNEUMONIAE IN RESPIRATORY SAMPLES IN BELGIUM QUESTIONING ITS ETIOLOGICAL ROLE IN RESPIRATORY TRACT INFECTIONS

被引：5

作者：

Padalko, E. ^{[1
]}

Boel, A. ^{[2
]}

Lagrou, K. ^{[3
,4
]}

Reynders, M. ^{[5
]}

China, B. ^{[6
]}

Vernelen, K. ^{[6
]}

机构：

[1] Ghent Univ Hosp, Dept Clin Chem Microbiol & Immunol, Ghent, Belgium

[2] OLV Aalst, Clin Microbiol, Aalst, Belgium

[3] Katholieke Univ Leuven, Dept Microbiol & Immunol, Louvain, Belgium

[4] UZ Leuven, Lab Med, Louvain, Belgium

[5] AZ Sint Jan Bruges Ostend AV, Lab Med Med Microbiol, Brugge, Belgium

[6] Sci Inst Publ Hlth, Qual Med Labs, Elsene, Belgium

来源：

ACTA CLINICA BELGICA | 2013年 / 68卷 / 03期

关键词：

Chlamydophila pneumoniae; respiratory; PCR; Belgium; laboratory diagnosis; COMMUNITY-ACQUIRED PNEUMONIA; CHLAMYDIA-PNEUMONIAE; PREVALENCE; GUIDELINES; MANAGEMENT; OUTBREAK; DISEASE; ADULTS; TWAR;

D O I：

10.2143/ACB.3241

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The actual burden of respiratory infections due to Chlamydophila pneumoniae is difficult to assess due to the major differences in positivity rates between PCR- and serology-based methods. The aim of the current study was to objectively analyse the yield of PCRs for the detection of C. pneumoniae in respiratory samples and to evaluate the additional value of performing laboratory diagnosis for C. pneumoniae in a setting of respiratory infection. The data based on routine analysis of respiratory samples with request for C. pneumoniae detection were collected from 4 large Belgian hospitals during 2 consecutive years. In total 3560 respiratory samples have been analysed and overall only 7 samples (0.2%) were found positive. Based on these observations, the critical evaluation of the actual role of C. pneumoniae in the etiology of lower respiratory infections and consequently of the extensive use of diagnostic tools for the detection of C. pneumoniae is needed.

引用

页码：166 / 168

页数：3

共 15 条

[1] Comparison of Real-Time PCR and a Microimmunofluorescence Serological Assay for Detection of Chlamydophila pneumoniae Infection in an Outbreak Investigation [J].

Benitez, Alvaro J. ;

Thurman, Kathleen A. ;

Diaz, Maureen H. ;

Conklin, Laura ;

Kendig, Newton E. ;

Winchell, Jonas M. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (01) :151-153

[2] Seasonal variations of 15 respiratory agents illustrated by the application of a multiplex polymerase chain reaction assay [J].

Brittain-Long, Robin ;

Andersson, Lars-Magnus ;

Olofsson, Sigvard ;

Lindh, Magnus ;

Westin, Johan .

SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES, 2012, 44 (01) :9-17

[3] Outbreak of Pneumonia in the Setting of Fatal Pneumococcal Meningitis among US Army Trainees: Potential Role of Chlamydia pneumoniae Infection [J].

Dawood, Fatimah S. ;

Ambrose, John F. ;

Russell, Bruce P. ;

Hawksworth, Anthony W. ;

Winchell, Jonas M. ;

Glass, Nina ;

Thurman, Kathleen ;

Soltis, Michele A. ;

McDonough, Erin ;

Warner, Agnes K. ;

Weston, Emily ;

Clemmons, Nakia S. ;

Rosen, Jennifer ;

Mitchell, Stephanie L. ;

Faix, Dennis J. ;

Blair, Patrick J. ;

Moore, Matthew R. ;

Lowery, John .

BMC INFECTIOUS DISEASES, 2011, 11

[4] Standardizing Chlamydia pneumoniae assays:: Recommendations from the Centers for Disease Control and Prevention (USA) and the Laboratory Centre for Disease Control (Canada) [J].

Dowell, SF ;

Peeling, RW ;

Boman, J ;

Carlone, GM ;

Fields, BS ;

Guarner, J ;

Hammerschlag, MR ;

Jackson, LA ;

Kuo, CC ;

Maass, M ;

Messmer, TO ;

Talkington, DF ;

Tondella, ML ;

Zaki, SR ;

Bandea, C ;

Black, C ;

O'Conner, S ;

Papp, J ;

Perilla, MJ ;

Schuchat, A ;

Stevens, V ;

Van Beneden, CA ;

Zell, ER ;

Cohen, C ;

Campbell, LA ;

Wwang, SP ;

Grayston, JT ;

Deal, CD ;

Gaydos, C ;

Schindler, L ;

Taylor, CE ;

Mahony, J ;

Fong, IW ;

Leinonen, M ;

Saikku, P ;

Maas, M ;

Ossewaarde, JM ;

Persson, K ;

Boman, J ;

Apfalter, P .

CLINICAL INFECTIOUS DISEASES, 2001, 33 (04) :492-502

[5] COMMUNITY-ACQUIRED AND HOSPITAL-ACQUIRED PNEUMONIA ASSOCIATED WITH CHLAMYDIA TWAR INFECTION DEMONSTRATED SEROLOGICALLY [J].

GRAYSTON, JT ;

DIWAN, VK ;

COONEY, M ;

WANG, SP .

ARCHIVES OF INTERNAL MEDICINE, 1989, 149 (01) :169-173

[6]

Kamata A, 2011, J INFECT CHEMOTHER

[7] Acute respiratory infection due to Chlamydia pneumoniae:: Current status of diagnostic methods [J].

Kumar, Swati ;

Hammerschlag, Margaret R. .

CLINICAL INFECTIOUS DISEASES, 2007, 44 (04) :568-576

[8] Prevalence of Chlamydia pneumoniae infection in Thai children with community-acquired pneumonia [J].

Likitnukul, S ;

Nunthapisud, P ;

Prapphal, N .

PEDIATRIC INFECTIOUS DISEASE JOURNAL, 2003, 22 (08) :749-750

[9] British Thoracic Society guidelines for the management of community acquired pneumonia in adults: update 2009 [J].

Lim, W. S. ;

Baudouin, S. V. ;

George, R. C. ;

Hill, A. T. ;

Jamieson, C. ;

Le Jeune, I. ;

Macfarlane, J. T. ;

Read, R. C. ;

Roberts, H. J. ;

Levy, M. L. ;

Wani, M. ;

Woodhead, M. A. .

THORAX, 2009, 64 :1-55

[10] Acute respiratory infection due to Mycoplasma pneumoniae: current status of diagnostic methods [J].

Loens, K. ;

Goossens, H. ;

Ieven, M. .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2010, 29 (09) :1055-1069

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