Aluminium chloride enhances colchicine production in root cultures of Gloriosa superba

被引：32

作者：

Ghosh, S

Ghosh, B

Jha, S

机构：

[1] Univ Calcutta, Ctr Adv Study Cell & Chromosome Res, Dept Bot, Kolkata 700019, W Bengal, India

[2] RKMVC Coll, Dept Bot, Rahara, W Bengal, India

来源：

BIOTECHNOLOGY LETTERS | 2006年 / 28卷 / 07期

关键词：

abiotic and biotic elicitors; colchicine; Gloriosa superba; root culture;

D O I：

10.1007/s10529-006-0004-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Root cultures of Gloriosa superba were treated with 5 mM methyl jasmonate and 125 mu M AlCl3 which enhanced the intracellular colchicine content of the roots by 50-fold and 63-fold, respectively. Ten millimolar of CaCl2 and 1 mM CdCl2 enhanced biomass significantly (7- to 8.6-fold, respectively) while maximum release of colchicine into the medium was obtained with 10 mM CdCl2. Casein hydrolysate, yeast extract and silver nitrate had no signi. cant effect on growth and colchicine accumulation in root cultures.

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收藏

页码：497 / 503

页数：7

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