cDNA cloning of a novel B subunit of Xenopus protein phosphatase 2A and its biological activity in oocytes

被引：7
作者：
Iwashita, J
Shima, H
Nagao, M
Sagata, N
机构：
[1] KYUSHU UNIV, FAC SCI, DEPT BIOL, FUKUOKA 812, JAPAN
[2] NATL CANC CTR, RES INST, DIV CARCINOGENESIS, TOKYO 104, JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 232卷 / 01期
关键词：
D O I：
10.1006/bbrc.1997.6259
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have cloned a cDNA encoding a novel B regulatory subunit of protein phosphatase 2A (PP2A) from a Xenopus oocyte cDNA library. The novel B subunit, termed B beta', shows the strongest overall sequence similarity to, but a distinct N-terminal sequence from, the beta isoform of the human/rat B subunit. When expressed ectopically in Xenopus oocytes, the B beta' isoform can augment the endogenous PP2A activity and inhibit oocyte maturation induced by progesterone. These results suggest that the B beta' isoform can form a complex with other PPBA subunits to make a trimeric PP2A holoenzyme in Xenopus oocytes and may negatively control the initiation of oocyte maturation. (C) 1997 Academic Press.
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收藏
页码：218 / 222
页数：5
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