Differentiation of Candida dubliniensis from Candida albicans by Means of MALDI-TOF Mass Spectrometry

被引：20
作者：
Hof, H. [1 ]
Eigner, U. [1 ]
Maier, T. [2 ]
Staib, P. [3 ]
机构：
[1] Lab Limbach, Heidelberg, Germany
[2] Bruker Daltonik GmbH, Bremen, Germany
[3] Hans Knoell Inst, Leibniz Inst Nat Prod Res & Infect Biol, Jena, Germany
来源：
CLINICAL LABORATORY | 2012年 / 58卷 / 9-10期
关键词：
Candida albicans; Candida dubliniensis; differentiation; MALDI-TOF; DESORPTION IONIZATION-TIME; IDENTIFICATION; PATHOGEN; SYSTEM;
D O I：
10.7754/Clin.Lab.2012.111213
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Background: Species specific differentiation of the two closely related yeasts, Candida albicans and C. dubliniensis, is difficult in routine diagnosis. Methods and Results: Here we show that MALDI-TOF MS is a practical and useful tool for the discrimination of C. albicans and C. dubliniensis, demonstrated by the analysis of reference strains from type culture collections and other well characterized isolates. The spectra of C. albicans and C. dubliniensis further revealed that each species consists of several clades. Conclusions: Reliable, routinely applicable methods for species specific differentiation of C albicans and C dubliniensis appear to be of particular importance to better understand the epidemiology and virulence of C. dubliniensis. (Clin. Lab. 2012;58:927-931. DOI: 10.7754/Clin.Lab.2012.111213)
引用
收藏
页码：927 / 931
页数：5
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