Genome editing in plants by engineered CRISPR-Cas9 recognizing NG PAM

被引：135

作者：

Endo, Masaki ^{[1
]}

Mikami, Masafumi ^{[1
,2
]}

Endo, Akira ^{[1
,6
]}

Kaya, Hidetaka ^{[1
,7
]}

Itoh, Takeshi ^{[3
]}

Nishimasu, Hiroshi ^{[4
]}

Nureki, Osamu ^{[4
]}

Toki, Seiichi ^{[1
,2
,5
]}

机构：

[1] Natl Agr & Food Res Org, Inst Agrobiol Sci, Plant Genome Engn Res Unit, Tsukuba, Ibaraki, Japan

[2] Yokohama City Univ, Grad Sch Nanobiosci, Yokohama, Kanagawa, Japan

[3] Natl Agr & Food Res Org, Adv Anal Ctr, Bioinformat Team, Tsukuba, Ibaraki, Japan

[4] Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Tokyo, Japan

[5] Yokohama City Univ, Kihara Inst Biol Res, Yokohama, Kanagawa, Japan

[6] Kaneka Corp, Biotechnol Dev Labs, Takasago, Hyogo, Japan

[7] Ehime Univ, Grad Sch Agr, Matsuyama, Ehime, Japan

来源：

NATURE PLANTS | 2019年 / 5卷 / 01期

关键词：

DNA; ENDONUCLEASE; RICE;

D O I：

10.1038/s41477-018-0321-8

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Streptococcus pyogenes Cas9 (SpCas9) is widely used for genome editing and requires NGG as a protospacer adjacent motif (PAM). Here, we show that the engineered SpCas9 (SpCas9-NGv1) can efficiently mutagenize endogenous target sites with NG PAMs in the rice and Arabidopsis genomes. Furthermore, we demonstrate that the SpCas9-NGv1 nickase fused to cytidine deaminase mediates C-to-T substitutions near the 5' end of the target sequence.

引用

页码：14 / 17

页数：4

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